Removal of the endothelial surface layer via hyaluronidase does not modulate monocyte and neutrophil interactions with the glomerular endothelium.
endothelium
glomerulonephritis
glomerulus
hyaluronan
inflammation
Journal
Microcirculation (New York, N.Y. : 1994)
ISSN: 1549-8719
Titre abrégé: Microcirculation
Pays: United States
ID NLM: 9434935
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
13
07
2023
received:
25
05
2023
accepted:
14
07
2023
pubmed:
26
7
2023
medline:
26
7
2023
entrez:
26
7
2023
Statut:
ppublish
Résumé
The endothelial surface layer (ESL), a layer of macromolecules on the surface of endothelial cells, can both impede and facilitate leukocyte recruitment. However, its role in monocyte and neutrophil recruitment in glomerular capillaries is unknown. We used multiphoton intravital microscopy to examine monocyte and neutrophil behavior in the glomerulus following ESL disruption with hyaluronidase. Constitutive retention and migration of monocytes and neutrophils within the glomerular microvasculature was unaltered by hyaluronidase. Consistent with this, inhibition of the hyaluronan-binding molecule CD44 also failed to modulate glomerular trafficking of these immune cells. To investigate the contribution of the ESL during acute inflammation, we induced glomerulonephritis via in situ immune complex deposition. This resulted in increases in glomerular retention of monocytes and neutrophils but did not induce marked reduction in the glomerular ESL. Furthermore, hyaluronidase treatment did not modify the prolonged retention of monocytes and neutrophils in the acutely inflamed glomerular microvasculature. These observations indicate that, despite evidence that the ESL has the capacity to inhibit leukocyte-endothelial cell interactions while also containing adhesive ligands for immune cells, neither of these functions modulate trafficking of monocytes and neutrophils in steady-state or acutely-inflamed glomeruli.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12823Subventions
Organisme : National Health and Medical Research Council
ID : 1064112
Organisme : National Health and Medical Research Council
ID : 1124459
Organisme : National Health and Medical Research Council
ID : 1042775
Informations de copyright
© 2023 The Authors. Microcirculation published by John Wiley & Sons Ltd.
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