Perturbed epigenetic transcriptional regulation in AML with IDH mutations causes increased susceptibility to NK cells.


Journal

Leukemia
ISSN: 1476-5551
Titre abrégé: Leukemia
Pays: England
ID NLM: 8704895

Informations de publication

Date de publication:
09 2023
Historique:
received: 23 01 2023
accepted: 10 07 2023
revised: 05 06 2023
medline: 28 8 2023
pubmed: 27 7 2023
entrez: 26 7 2023
Statut: ppublish

Résumé

Isocitrate dehydrogenase (IDH) mutations are found in 20% of acute myeloid leukemia (AML) patients. However, only 30-40% of the patients respond to IDH inhibitors (IDHi). We aimed to identify a molecular vulnerability to tailor novel therapies for AML patients with IDH mutations. We characterized the transcriptional and epigenetic landscape with the IDH2i AG-221, using an IDH2 mutated AML cell line model and AML patient cohorts, and discovered a perturbed transcriptional regulatory network involving myeloid transcription factors that were partly restored after AG-221 treatment. In addition, hypermethylation of the HLA cluster caused a down-regulation of HLA class I genes, triggering an enhanced natural killer (NK) cell activation and an increased susceptibility to NK cell-mediated responses. Finally, analyses of DNA methylation data from IDHi-treated patients showed that non-responders still harbored hypermethylation in HLA class I genes. In conclusion, this study provides new insights suggesting that IDH mutated AML is particularly sensitive to NK cell-based personalized immunotherapy.

Identifiants

pubmed: 37495775
doi: 10.1038/s41375-023-01972-3
pii: 10.1038/s41375-023-01972-3
pmc: PMC10457197
doi:

Substances chimiques

enasidenib 3T1SS4E7AG
Isocitrate Dehydrogenase EC 1.1.1.41

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1830-1841

Informations de copyright

© 2023. The Author(s).

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Auteurs

Anna Palau (A)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Filip Segerberg (F)

Center for Hematology and Regenerative Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.

Michael Lidschreiber (M)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

Katja Lidschreiber (K)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Aonghus J Naughton (AJ)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Maria Needhamsen (M)

Department of Clinical Neuroscience, Center for Molecular Medicine, Karolinska Institutet, Solna, Sweden.

Lisa Anna Jung (LA)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Maja Jagodic (M)

Department of Clinical Neuroscience, Center for Molecular Medicine, Karolinska Institutet, Solna, Sweden.

Patrick Cramer (P)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.
Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

Sören Lehmann (S)

Center for Hematology and Regenerative Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden. soren.lehmann@ki.se.
Hematology Centre, Karolinska University Hospital, Stockholm, Sweden. soren.lehmann@ki.se.
Hematology Unit, Department of Medical Sciences, Uppsala University, Uppsala, Sweden. soren.lehmann@ki.se.

Mattias Carlsten (M)

Center for Hematology and Regenerative Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden. mattias.carlsten@ki.se.
Center for Cell Therapy and Allogeneic Stem Cell Transplantation, Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, Sweden. mattias.carlsten@ki.se.

Andreas Lennartsson (A)

Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden. andreas.lennartsson@ki.se.

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