AraC-induced neuron-like differentiation of human NTERA2/D1 cells and quantification of endogenous pre-mir-106b and 19b levels.
Journal
microPublication biology
ISSN: 2578-9430
Titre abrégé: MicroPubl Biol
Pays: United States
ID NLM: 101759238
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
03
2023
revised:
24
05
2023
accepted:
11
07
2023
medline:
27
7
2023
pubmed:
27
7
2023
entrez:
27
7
2023
Statut:
epublish
Résumé
MicroRNAs (miRNAs) are approximately 22 nucleotide-long non-coding RNAs that are encoded in the genome. miRNAs form base pairs with target mRNAs in the RNA-induced silencing complex and repress their expression through a mechanism called RNA silencing. Expression profiles of miRNAs differ between cells and tissues. In this study, we performed cytosine β-D-arabinofuranoside (AraC)-induced neuron-like differentiation of human NTERA2/D1 (NT2) cells and quantified endogenous miRNA levels using quantitative RT-PCR. In conclusion, pre-mir-106b and pre-mir-19b levels were decreased after AraC-induced neuron-like differentiation of NT2 cells, indicating the functional relevance of miRNAs in the differentiation of mammalian cells.
Identifiants
pubmed: 37497181
doi: 10.17912/micropub.biology.000803
pmc: PMC10366682
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright: © 2023 by the authors.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest present.
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