Prevalence of blood and skin trypanosomes in domestic and wild fauna from two sleeping sickness foci in Southern Cameroon.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
received:
01
03
2023
accepted:
12
07
2023
revised:
08
08
2023
medline:
9
8
2023
pubmed:
27
7
2023
entrez:
27
7
2023
Statut:
epublish
Résumé
Although studies on African Trypanosomiases revealed a variety of trypanosome species in the blood of various animal taxa, animal reservoirs of Trypanosoma brucei gambiense and anatomical niches such as skin have been overlooked in most epidemiological settings. This study aims to update epidemiological data on trypanosome infections in animals from human African trypanosomiasis (HAT) foci of Cameroon. Blood and skin snips were collected from 291 domestic and wild animals. DNA was extracted from blood and skin snips and molecular approaches were used to identify different trypanosomes species. Immunohistochemical analyses were used to confirm trypanosome infections in skin snips. PCR revealed 137 animals (47.1%) with at least one trypanosome species in the blood and/or in the skin. Of these 137 animals, 90 (65.7%) and 32 (23.4%) had trypanosome infections respectively in the blood and skin. Fifteen (10.9%) animals had trypanosome infections in both blood and skin snip. Animals from the Campo HAT focus (55.0%) were significantly (X2 = 17.6; P< 0.0001) more infected than those (29.7%) from Bipindi. Trypanosomes of the subgenus Trypanozoon were present in 27.8% of animals while T. vivax, T. congolense forest type and savannah type were detected in 16.5%, 10.3% and 1.4% of animals respectively. Trypanosoma b. gambiense infections were detected in the blood of 7.6% (22/291) of animals. No T. b. gambiense infection was detected in skin. This study highlights the presence of several trypanosome species in the blood and skin of various wild and domestic animals. Skin appeared as an anatomical reservoir for trypanosomes in animals. Despite methodological limitations, pigs, sheep, goats and wild animals were confirmed as potential reservoirs of T. b. gambiense. These animal reservoirs must be considered for the designing of control strategies that will lead to sustainable elimination of HAT.
Identifiants
pubmed: 37498955
doi: 10.1371/journal.pntd.0011528
pii: PNTD-D-23-00286
pmc: PMC10411957
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0011528Subventions
Organisme : Department of Health
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
Copyright: © 2023 Magang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that they have no competing interests.
Références
Int J Parasitol. 1992 Nov;22(7):909-18
pubmed: 1459784
PLoS Negl Trop Dis. 2021 Dec 22;15(12):e0010036
pubmed: 34937054
PLoS Negl Trop Dis. 2017 Oct 18;11(10):e0005993
pubmed: 29045405
Trop Med Int Health. 2009 May;14(5):535-41
pubmed: 19320872
Parasit Vectors. 2019 Aug 27;12(1):420
pubmed: 31455430
Microbes Infect. 2012 Jul;14(7-8):651-8
pubmed: 22387499
Parasitology. 1989 Aug;99 Pt 1:57-66
pubmed: 2797872
Arch Dermatol. 1995 Oct;131(10):1178-82
pubmed: 7574836
Infect Genet Evol. 2008 Jan;8(1):34-9
pubmed: 17977803
Eur J Biochem. 1988 Oct 1;176(3):527-34
pubmed: 2458923
Korean J Parasitol. 2019 Apr;57(2):191-195
pubmed: 31104413
PLoS Negl Trop Dis. 2020 Apr 7;14(4):e0007737
pubmed: 32255793
PLoS Pathog. 2021 Sep 16;17(9):e1009866
pubmed: 34529724
Nature. 1978 Apr 13;272(5654):613-4
pubmed: 643055
Infect Genet Evol. 2008 Dec;8(6):847-54
pubmed: 18790085
Parasite. 2011 May;18(2):171-9
pubmed: 21678793
Med Vet Entomol. 2022 Sep;36(3):260-268
pubmed: 35593526
Trends Parasitol. 2017 Jul;33(7):499-509
pubmed: 28456474
Am J Trop Med Hyg. 2002 Sep;67(3):289-95
pubmed: 12408669
PLoS Pathog. 2016 Jul 21;12(7):e1005744
pubmed: 27441553
Acta Trop. 1981 Mar;38(1):15-28
pubmed: 6111913
Acta Trop. 2004 Oct;92(2):139-46
pubmed: 15350866
Trends Parasitol. 2018 Mar;34(3):197-207
pubmed: 29396200
Lancet. 2017 Nov 25;390(10110):2397-2409
pubmed: 28673422
Parasite. 2002 Dec;9(4):345-9
pubmed: 12514949
Infect Genet Evol. 2013 Aug;18:66-73
pubmed: 23624186
PLoS Negl Trop Dis. 2012;6(12):e1949
pubmed: 23272259
Clin Infect Dis. 2021 Jul 1;73(1):12-20
pubmed: 32638003
Infect Immun. 1993 Nov;61(11):4540-5
pubmed: 8406850
Acta Trop. 2006 May;98(2):183-8
pubmed: 16723098
Parasit Vectors. 2008 Feb 12;1(1):3
pubmed: 18275594
Parasit Vectors. 2014 Aug 16;7:374
pubmed: 25129168
Elife. 2016 Sep 22;5:
pubmed: 27653219
Front Immunol. 2020 Jun 12;11:1250
pubmed: 32595652
Infect Genet Evol. 2006 Mar;6(2):147-53
pubmed: 16236560
PLoS Negl Trop Dis. 2022 Jan 18;16(1):e0010047
pubmed: 35041668
Trends Parasitol. 2009 Mar;25(3):132-8
pubmed: 19200783
Parasite. 2020;27:63
pubmed: 33206595
Front Microbiol. 2015 Jul 24;6:765
pubmed: 26257727
Vet Parasitol. 2006 Jun 30;139(1-3):57-66
pubmed: 16567049
Parasite. 2010 Mar;17(1):61-6
pubmed: 20387740