Inhibiting mechanism of Alpiniae oxyphyllae fructus polysaccharide 3 against the replication of porcine epidemic diarrhea virus.

Animals Swine Cell Line Porcine epidemic diarrhea virus / physiology Heterogeneous Nuclear Ribonucleoprotein A1 RNA-Dependent RNA Polymerase Coronavirus Infections Swine Diseases / drug therapy Virus Replication
Alpiniae oxyphyllae fructus polysaccharide 3 Heterogeneous nuclear ribonucleoprotein A1 Porcine epidemic diarrhea virus RNA-Dependent RNA polymerase Viral replication

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
10 2023
Historique:
received: 15 03 2023
revised: 17 07 2023
accepted: 20 07 2023
medline: 9 10 2023
pubmed: 28 7 2023
entrez: 27 7 2023
Statut: ppublish

Résumé

Porcine epidemic diarrhea virus (PEDV) causes diarrhea, vomiting, and death in piglets. Our previous study has revealed the anti-PEDV activity of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3). However, it is still unknown whether AOFP3 can inhibit the replication of PEDV. Therefore, the effect of AOFP3 on PEDV replication was investigated in the present study, along with analysis of viral RdRp activity and expression of hnRNP A1 by RNA polymerase activity assay in vitro, RIP assay, and Western blotting. The results showed that both the PEDV gene and protein levels in IPEC-J2 cells decreased with AOFP3 treatment. In addition, AOFP3 significantly reduced PEDV's replication by down-regulating the activity of PEDV RdRp and reducing the expression of hnRNP A1, whereas only the bind of RdRp to PEDV 3'UTR was inhibited in AOFP3 treated cells.

Identifiants

DOI: 10.1016/j.virol.2023.109848 PMID: 37499528
pubmed: 37499528
pii: S0042-6822(23)00161-7
doi: 10.1016/j.virol.2023.109848
pii:
doi:

Substances chimiques

Heterogeneous Nuclear Ribonucleoprotein A1 0
RNA-Dependent RNA Polymerase EC 2.7.7.48

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109848

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Haowen Wu (H)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.

Qiyuan Luo (Q)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.

Yijing Chen (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.

Suya Liao (S)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.

Huricha Chen (H)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China.

Yun Chen (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China. Electronic address: ychen@hainanu.edu.cn.

Yao Qin (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, School of Animal Science and Technology of Hainan University, Haikou, 570228, PR China. Electronic address: qinyao@hainanu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Alphacoronavirus › Virus de la diarrhée porcine épidémique › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Protéines à motif de reconnaissance de l'ARN › Ribonucléoprotéines nucléaires hétérogènes › Ribonucléoprotéine nucléaire hétérogène du groupe A-B › Ribonucléoprotéine nucléaire hétérogène A1 › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › RNA replicase › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ARN › Infections à Nidovirales › Infections à Coronaviridae › Infections à coronavirus › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Maladies de l'animal › Maladies des porcs › Inhibiting mechanism of Alpiniae oxyphyllae...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Inhibiting mechanism of Alpiniae oxyphyllae...