A transcriptional and regulatory map of mouse somite maturation.
ATAC-seq
RNA-seq
chromatin
gene regulation
mouse development
somite maturation
somites
transcription
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
09 10 2023
09 10 2023
Historique:
received:
25
01
2023
revised:
12
05
2023
accepted:
04
07
2023
medline:
12
10
2023
pubmed:
28
7
2023
entrez:
27
7
2023
Statut:
ppublish
Résumé
The mammalian body plan is shaped by rhythmic segmentation of mesoderm into somites, which are transient embryonic structures that form down each side of the neural tube. We have analyzed the genome-wide transcriptional and chromatin dynamics occurring within nascent somites, from early inception of somitogenesis to the latest stages of body plan establishment. We created matched gene expression and open chromatin maps for the three leading pairs of somites at six time points during mouse embryonic development. We show that the rate of somite differentiation accelerates as development progresses. We identified a conserved maturation program followed by all somites, but somites from more developed embryos concomitantly switch on differentiation programs from derivative cell lineages soon after segmentation. Integrated analysis of the somitic transcriptional and chromatin activities identified opposing regulatory modules controlling the onset of differentiation. Our results provide a powerful, high-resolution view of the molecular genetics underlying somitic development in mammals.
Identifiants
pubmed: 37499658
pii: S1534-5807(23)00333-7
doi: 10.1016/j.devcel.2023.07.003
pmc: PMC10563765
pii:
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1983-1995.e7Subventions
Organisme : Cancer Research UK
ID : 20412
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 22398
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108438/Z/15
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 202878/Z/16/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests J.C.M. has been an employee of Genentech since September 2022.
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