High-resolution photocatalytic mapping of SARS-CoV-2 spike interactions on the cell surface.
SARS-CoV-2
photocatalysis
proximity labeling
viral entry
viral-host interactome
Journal
Cell chemical biology
ISSN: 2451-9448
Titre abrégé: Cell Chem Biol
Pays: United States
ID NLM: 101676030
Informations de publication
Date de publication:
19 10 2023
19 10 2023
Historique:
received:
04
10
2022
revised:
03
05
2023
accepted:
29
06
2023
medline:
27
10
2023
pubmed:
28
7
2023
entrez:
27
7
2023
Statut:
ppublish
Résumé
Identifying virus-host interactions on the cell surface can improve our understanding of viral entry and pathogenesis. SARS-CoV-2, the causative agent of the COVID-19 disease, uses ACE2 as a receptor to enter cells. Yet the full repertoire of cell surface proteins that contribute to viral entry is unknown. We developed a photocatalyst-based viral-host protein microenvironment mapping platform (ViraMap) to probe the molecular neighborhood of the SARS-CoV-2 spike protein on the human cell surface. Application of ViraMap to ACE2-expressing cells captured ACE2, the established co-receptor NRP1, and several novel cell surface proteins. We systematically analyzed the relevance of these candidate proteins to SARS-CoV-2 entry by knockdown and overexpression approaches in pseudovirus and authentic infection models and identified PTGFRN and EFNB1 as bona fide viral entry factors. Our results highlight additional host targets that participate in SARS-CoV-2 infection and showcase ViraMap as a powerful platform for defining viral interactions on the cell surface.
Identifiants
pubmed: 37499664
pii: S2451-9456(23)00207-6
doi: 10.1016/j.chembiol.2023.06.028
pii:
doi:
Substances chimiques
spike protein, SARS-CoV-2
0
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Spike Glycoprotein, Coronavirus
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1313-1322.e7Informations de copyright
Copyright © 2023 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests T.R.-R., K.A.R., T.J.B., J.M.B., C.H.W., D.J.H., K.A.V., E.C.H., O.O.F., and R.C.O. were employed by Merck & Co. during the experimental planning, execution, and preparation of this manuscript. Other authors declare no competing interests.