Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.
T-DNA insertions
genetically engineered crops.
regulation of GM crops
target capture sequencing
transgenic events
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2023
2023
Historique:
received:
01
02
2023
accepted:
15
06
2023
medline:
28
7
2023
pubmed:
28
7
2023
entrez:
28
7
2023
Statut:
epublish
Résumé
The commercialization of GE crops requires a rigorous safety assessment, which includes a precise DNA level characterization of inserted T-DNA. In the past, several strategies have been developed for identifying T-DNA insertion sites including, Southern blot and different PCR-based methods. However, these methods are often challenging to scale up for screening of dozens of transgenic events and for crops with complex genomes, like potato. Here, we report using target capture sequencing (TCS) to characterize the T-DNA structure and insertion sites of 34 transgenic events in potato. This T-DNA is an 18 kb fragment between left and right borders and carries three resistance (R) genes (
Identifiants
pubmed: 37502707
doi: 10.3389/fpls.2023.1156665
pmc: PMC10369180
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1156665Informations de copyright
Copyright © 2023 Magembe, Li, Taheri, Zhou and Ghislain.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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