Upscaled Skeletal Muscle Engineered Tissue with In Vivo Vascularization and Innervation Potential.
SVF cells
innervation
perfusion-bioreactor
scale-up
skeletal myoblasts
tissue engineering
vascularization
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
04 Jul 2023
04 Jul 2023
Historique:
received:
08
05
2023
revised:
13
06
2023
accepted:
21
06
2023
medline:
29
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
Engineering functional tissues of clinically relevant size (in mm-scale) in vitro is still a challenge in tissue engineering due to low oxygen diffusion and lack of vascularization. To address these limitations, a perfusion bioreactor was used to generate contractile engineered muscles of a 3 mm-thickness and a 8 mm-diameter. This study aimed to upscale the process to 50 mm in diameter by combining murine skeletal myoblasts (SkMbs) with human adipose-derived stromal vascular fraction (SVF) cells, providing high neuro-vascular potential in vivo. SkMbs were cultured on a type-I-collagen scaffold with (co-culture) or without (monoculture) SVF. Large-scale muscle-like tissue showed an increase in the maturation index over time (49.18 ± 1.63% and 76.63 ± 1.22%, at 9 and 11 days, respectively) and a similar force of contraction in mono- (43.4 ± 2.28 µN) or co-cultured (47.6 ± 4.7 µN) tissues. Four weeks after implantation in subcutaneous pockets of nude rats, the vessel length density within the constructs was significantly higher in SVF co-cultured tissues (5.03 ± 0.29 mm/mm
Identifiants
pubmed: 37508827
pii: bioengineering10070800
doi: 10.3390/bioengineering10070800
pmc: PMC10376693
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Swiss National Science Foundation
ID : 310030_172989
Pays : Switzerland
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