Methadone Potentiates the Cytotoxicity of Temozolomide by Impairing Calcium Homeostasis and Dysregulation of PARP in Glioblastoma Cells.
apoptosis
glioblastoma
methadone
oxidative stress
temozolomide
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
11 Jul 2023
11 Jul 2023
Historique:
received:
26
12
2022
revised:
30
06
2023
accepted:
08
07
2023
medline:
29
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
Methadone is commonly used as an alternative to morphine in patients with pain associated with glioblastoma and other cancers. Although concomitant administration of methadone and cytostatics is relatively common, the effect of methadone on the efficacy of cytostatic drugs has not been well studied until recently. Moreover, the mechanism behind the effect of methadone on temozolomide efficacy has not been investigated in previous studies, or this effect has been automatically attributed to opioid receptors. Our findings indicate that methadone potentiates the effect of temozolomide on rat C6 glioblastoma cells and on human U251 and T98G glioblastoma cells and increases cell mortality by approximately 50% via a mechanism of action independent of opioid receptors. Our data suggest that methadone acts by affecting mitochondrial potential, the level of oxidative stress, intracellular Ca
Identifiants
pubmed: 37509230
pii: cancers15143567
doi: 10.3390/cancers15143567
pmc: PMC10377588
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Charles University
ID : SVV-260571/2020
Organisme : Charles University
ID : 1722218
Références
Neoplasia. 2014 Oct 23;16(10):874-82
pubmed: 25379024
Expert Rev Anticancer Ther. 2001 Jun;1(1):13-9
pubmed: 12113120
MethodsX. 2020 May 28;7:100938
pubmed: 32551241
Cell Commun Signal. 2010 Dec 22;8:31
pubmed: 21176168
Oncotarget. 2013 May;4(5):677-90
pubmed: 23633472
CNS Oncol. 2018 Jul 1;7(3):CNS18
pubmed: 29916277
Am J Physiol Cell Physiol. 2004 Oct;287(4):C817-33
pubmed: 15355853
PLoS One. 2011;6(9):e24665
pubmed: 21931801
J Neurochem. 2005 Sep;94(5):1329-41
pubmed: 16000156
Front Oncol. 2014 Nov 17;4:302
pubmed: 25478322
J Neurol Sci. 2016 Aug 15;367:101-6
pubmed: 27423571
Cancer Res. 2008 Aug 1;68(15):6059-64
pubmed: 18676827
Int J Med Sci. 2020 Oct 16;17(17):2831-2843
pubmed: 33162811
Cell Death Differ. 2016 Jun;23(6):952-61
pubmed: 26891692
Int J Mol Sci. 2021 Nov 03;22(21):
pubmed: 34769368
Curr Mol Pharmacol. 2012 Jan;5(1):102-14
pubmed: 22122467
Physiol Rev. 2000 Jan;80(1):315-60
pubmed: 10617771
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9921-5
pubmed: 2602382
Apoptosis. 2005 Jan;10(1):209-17
pubmed: 15711937
Oncotarget. 2017 Aug 11;8(40):69185-69197
pubmed: 28978189
Neuro Oncol. 2021 Jun 1;23(6):920-931
pubmed: 33433610
Pharmacol Res. 2002 Jun;45(6):469-73
pubmed: 12162947
Eur J Pain. 2007 Nov;11(8):823-30
pubmed: 17331764
Int J Cell Biol. 2012;2012:642482
pubmed: 22778742
Int J Cancer. 2018 Oct 15;143(8):1840-1848
pubmed: 29516505
Biochemistry (Mosc). 2016 Feb;81(2):91-100
pubmed: 27260389
CNS Neurosci Ther. 2012 Jul;18(7):536-46
pubmed: 22530672
Mol Clin Oncol. 2013 Nov;1(6):935-941
pubmed: 24649273
Med Hypotheses. 2014 Aug;83(2):175-81
pubmed: 24907229
Eur J Anaesthesiol. 2020 Oct;37(10):934-943
pubmed: 32516227
Int J Mol Sci. 2020 Sep 23;21(19):
pubmed: 32977591
Mitochondrion. 2020 May;52:56-66
pubmed: 32045717
J Biol Chem. 2010 Dec 17;285(51):39759-67
pubmed: 20870728
Sci Rep. 2017 Oct 9;7(1):12876
pubmed: 28993682
Pharmacol Res. 2011 Apr;63(4):352-60
pubmed: 21145398
Cell Cycle. 2014;13(10):1560-70
pubmed: 24626197
Arch Intern Med. 2004 Feb 9;164(3):277-88
pubmed: 14769623
Anticancer Res. 2019 Jul;39(7):3633-3639
pubmed: 31262889