Characterization of Mitochondrial DNA Methylation of Alzheimer's Disease in Plasma Cell-Free DNA.
Alzheimer’s disease
cell-free DNA
mitochondrial DNA methylation
noninvasive diagnosis
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
12 Jul 2023
12 Jul 2023
Historique:
received:
17
05
2023
revised:
29
06
2023
accepted:
10
07
2023
medline:
29
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
Noninvasive diagnosis of Alzheimer's disease (AD) is important for patients. Significant differences in the methylation of mitochondrial DNA (mtDNA) were found in AD brain tissue. Cell-free DNA (cfDNA) is a noninvasive and economical diagnostic tool. We aimed to characterize mtDNA methylation alterations in the plasma cfDNA of 31 AD patients and 26 age- and sex-matched cognitively normal control subjects. We found that the mtDNA methylation patterns differed between AD patients and control subjects. The mtDNA was predominantly hypomethylated in the plasma cfDNA of AD patients. The hypomethylation sites or regions were mainly located in mt-rRNA, mt-tRNA, and D-Loop regions. The hypomethylation of the D-Loop region in plasma cfDNA of AD patients was consistent with that in previous studies. This study presents evidence that hypomethylation in the non-protein coding region of mtDNA may contribute to the pathogenesis of AD and potential application for the diagnosis of AD.
Identifiants
pubmed: 37510095
pii: diagnostics13142351
doi: 10.3390/diagnostics13142351
pmc: PMC10378411
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Natural Science Foundation of Hunan Province
ID : 2022JJ30694
Organisme : Central South University Innovation-Driven Research Programme
ID : 2023CXQD066
Organisme : Hunan Provincial Maternal and Child Health Care Hospital
ID : KF2021001
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