Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells.
Kunjin virus
RNA-Seq
SILAC
Zika virus
alternative splicing
flavivirus
proteomics
transcriptomics
virus–host interaction
yellow fever virus
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
23 06 2023
23 06 2023
Historique:
received:
26
05
2023
revised:
20
06
2023
accepted:
22
06
2023
medline:
31
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
As obligate intracellular parasites, viruses rely heavily on host cells for replication, and therefore dysregulate several cellular processes for their benefit. In return, host cells activate multiple signaling pathways to limit viral replication and eradicate viruses. The present study explores the complex interplay between viruses and host cells through next generation RNA sequencing as well as mass spectrometry (SILAC). Both the coding transcriptome and the proteome of human brain-derived U87 cells infected with Kunjin virus, Zika virus, or Yellow Fever virus were compared to the transcriptome and the proteome of mock-infected cells. Changes in the abundance of several hundred mRNAs and proteins were found in each infection. Moreover, the alternative splicing of hundreds of mRNAs was found to be modulated upon viral infection. Interestingly, a significant disconnect between the changes in the transcriptome and those in the proteome of infected cells was observed. These findings provide a global view of the coding transcriptome and the proteome of Flavivirus-infected cells, leading to a better comprehension of Flavivirus-host interactions.
Identifiants
pubmed: 37515107
pii: v15071419
doi: 10.3390/v15071419
pmc: PMC10385720
pii:
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI132668
Pays : United States
Organisme : NIH HHS
ID : R01 AI132668
Pays : United States
Références
Curr Opin Virol. 2016 Oct;20:119-128
pubmed: 27792906
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
Front Immunol. 2018 Sep 26;9:2180
pubmed: 30319635
Eur J Neurol. 2022 Sep;29(9):2823-2831
pubmed: 35699338
Cell Stem Cell. 2016 May 5;18(5):587-90
pubmed: 26952870
J Proteome Res. 2013 Jun 7;12(6):2666-78
pubmed: 23647205
Semin Cell Dev Biol. 2005 Feb;16(1):13-20
pubmed: 15659335
Nucleic Acids Res. 2020 Jul 9;48(12):6824-6838
pubmed: 32432721
J Neurosci. 2003 Jan 15;23(2):568-78
pubmed: 12533617
N Engl J Med. 2016 May 19;374(20):1981-7
pubmed: 27074377
Viruses. 2020 Sep 04;12(9):
pubmed: 32899736
Nucleic Acids Res. 2023 Jan 6;51(D1):D1539-D1548
pubmed: 36370099
J Proteome Res. 2007 Dec;6(12):4592-600
pubmed: 17979228
Genesis. 2021 Sep;59(9):e23442
pubmed: 34402594
PLoS Negl Trop Dis. 2013;7(3):e2107
pubmed: 23516652
Indian J Med Res. 2021 Mar;154(3):433-445
pubmed: 35345069
Am J Trop Med Hyg. 2019 Sep;101(3):484-490
pubmed: 31333155
PLoS Pathog. 2012;8(6):e1002741
pubmed: 22761570
BMC Genomics. 2008 Oct 16;9:488
pubmed: 18925949
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Sci Transl Med. 2016 Aug 31;8(354):354re3
pubmed: 27582061
BMC Bioinformatics. 2015 May 22;16:169
pubmed: 25994840
Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17345-50
pubmed: 20855599
Microbes Infect. 2004 Jun;6(7):702-13
pubmed: 15158778
Arch Med Sci. 2015 Dec 10;11(6):1164-78
pubmed: 26788077
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
PLoS Pathog. 2012;8(3):e1002584
pubmed: 22457619
Genome Biol. 2007;8(2):R19
pubmed: 17291332
Emerg Microbes Infect. 2017 Apr 26;6(4):e24
pubmed: 28442752
Nat Microbiol. 2020 Jun;5(6):796-812
pubmed: 32367055
PLoS Pathog. 2016 Aug 30;12(8):e1005841
pubmed: 27575636
Annu Rev Biochem. 2015;84:291-323
pubmed: 25784052
EBioMedicine. 2023 Mar;89:104457
pubmed: 36739631
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Biochimie. 2007 Jun-Jul;89(6-7):799-811
pubmed: 17451862
Viruses. 2013 Jul 08;5(7):1664-81
pubmed: 23881275
J Neurovirol. 2014 Dec;20(6):539-60
pubmed: 25287260
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552
pubmed: 34723319
Proc Natl Acad Sci U S A. 2007 May 22;104(21):9041-6
pubmed: 17502609
Mol Cell Proteomics. 2017 Oct;16(10):1850-1863
pubmed: 28747380
J Biol Chem. 2019 Nov 1;294(44):16282-16296
pubmed: 31519749
Viruses. 2020 May 05;12(5):
pubmed: 32380717
Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):E5593-601
pubmed: 25480548
Curr Opin Virol. 2016 Apr;17:1-10
pubmed: 26426962
RNA. 2012 Nov;18(11):2029-40
pubmed: 23006624
Dev Biol. 2005 Jul 15;283(2):437-45
pubmed: 15949792
J Virol. 2018 Nov 12;92(23):
pubmed: 30232189
Glia. 2023 Aug;71(8):1791-1803
pubmed: 36866453