Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris.

Behavior Evolutionary Biology Neuroscience

Journal

STAR protocols

ISSN: 2666-1667

Titre abrégé: STAR Protoc

Pays: United States

ID NLM: 101769501

Informations de publication

Date de publication:
28 Jul 2023

Historique:

received: 04 04 2023

revised: 07 05 2023

accepted: 21 06 2023

medline: 29 7 2023

pubmed: 29 7 2023

entrez: 29 7 2023

Statut: aheadofprint

Résumé

The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.

Identifiants

DOI: 10.1016/j.xpro.2023.102453 PMID: 37515760 PMC: PMC10400962

pubmed: 37515760

pii: S2666-1667(23)00420-3

doi: 10.1016/j.xpro.2023.102453

pmc: PMC10400962

pii:

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

102453

Informations de copyright

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing financial interests.

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