Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons.
Cell Differentiation
Neuroscience
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
28 Jul 2023
28 Jul 2023
Historique:
received:
24
03
2023
revised:
11
07
2023
accepted:
12
07
2023
medline:
29
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
aheadofprint
Résumé
Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks. For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).
Identifiants
pubmed: 37515763
pii: S2666-1667(23)00453-7
doi: 10.1016/j.xpro.2023.102486
pmc: PMC10400954
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102486Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Toxicol In Vitro. 2003 Jun;17(3):367-73
pubmed: 12781215
Cell Rep. 2012 Jun 28;1(6):703-14
pubmed: 22813745
Nat Med. 2006 Nov;12(11):1259-68
pubmed: 17057709
Stem Cell Reports. 2021 Jul 13;16(7):1763-1776
pubmed: 34171286
Nat Protoc. 2006;1(1):241-5
pubmed: 17406239
Mol Neurobiol. 2017 Mar;54(2):1119-1128
pubmed: 26803497
Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12524-12533
pubmed: 31160442
Sci Rep. 2022 Oct 13;12(1):17176
pubmed: 36229560
Elife. 2021 Jan 20;10:
pubmed: 33470930
Cell Rep. 2018 May 22;23(8):2509-2523
pubmed: 29791859
J Clin Invest. 2012 May;122(5):1653-66
pubmed: 22523069
Nat Protoc. 2012 Oct;7(10):1836-46
pubmed: 22976355
Neuron. 2013 Jun 5;78(5):785-98
pubmed: 23764284
Stem Cell Res. 2021 Apr;52:102240
pubmed: 33610017
Lancet. 2021 Jun 12;397(10291):2284-2303
pubmed: 33848468
Stem Cell Res. 2019 Dec;41:101617
pubmed: 31731178
Biochem Biophys Res Commun. 2019 Aug 13;516(1):222-228
pubmed: 31208718
PLoS Biol. 2022 Mar 9;20(3):e3001578
pubmed: 35263320
Front Cell Dev Biol. 2020 Nov 05;8:578907
pubmed: 33224948
J Parkinsons Dis. 2022;12(3):871-884
pubmed: 34958047
Stem Cells Transl Med. 2019 Feb;8(2):112-123
pubmed: 30387318