Leishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepal.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 07 2023
29 07 2023
Historique:
received:
18
02
2023
accepted:
22
06
2023
medline:
31
7
2023
pubmed:
30
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
Cutaneous leishmaniasis cases have increased dramatically in recent years in Nepal. The study offers molecular identification of the Leishmania species using 40 patient's aspiration biopsy samples, targeting markers kinetoplast minicircle DNA (kDNA) and internal transcribed spacer-1 (ITS1). Among molecularly diagnosed 22 cutaneous leishmaniasis cases, L. donovani complex was identified in 13 instances and L. major in 9 cases. The ITS1 PCR was positive in 12 of the positive nested- kDNA PCR cases (12/22), confirming L. donovani complex in seven of the cases and L. major in five of the cases. In addition, the study conclude that concurrent occurrence of atypical cutaneous infections caused by L. donovani parasite in 59.1% of cases and typical cutaneous infections caused by L. major parasite in 40.9% of cases. A Phylogentic analaysis showed that the detected L. donovani species present null genetic distances from seven references of L. donovani, but slight differences between ITS1 sequences and not grouped into a significant monophyletic cluster.
Identifiants
pubmed: 37516780
doi: 10.1038/s41598-023-37458-6
pii: 10.1038/s41598-023-37458-6
pmc: PMC10387047
doi:
Substances chimiques
DNA, Kinetoplast
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12329Informations de copyright
© 2023. The Author(s).
Références
BMC Infect Dis. 2019 May 21;19(1):444
pubmed: 31113385
Am J Trop Med Hyg. 2007 May;76(5):896-901
pubmed: 17488912
Acta Trop. 2022 Aug;232:106512
pubmed: 35568069
J Parasit Dis. 2020 Dec;44(4):781-784
pubmed: 33184545
Trop Med Health. 2021 Sep 9;49(1):72
pubmed: 34503578
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
Mem Inst Oswaldo Cruz. 1991 Jan-Mar;86(1):51-6
pubmed: 1842401
Emerg Infect Dis. 2007 Mar;13(3):476-8
pubmed: 17552105
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Am Fam Physician. 2004 Mar 15;69(6):1455-60
pubmed: 15053410
J Clin Microbiol. 2007 Jul;45(7):2110-5
pubmed: 17475750
PLoS Negl Trop Dis. 2018 Sep 27;12(9):e0006659
pubmed: 30260957
iScience. 2022 Jan 22;25(2):103802
pubmed: 35198868
J Clin Microbiol. 1998 Oct;36(10):2877-81
pubmed: 9738037
Emerg Infect Dis. 2010 Feb;16(2):231-7
pubmed: 20113552
Parasitol Int. 2020 Feb;74:101991
pubmed: 31520692
Heliyon. 2021 Jun 11;7(6):e07282
pubmed: 34189318
J Clin Microbiol. 2006 Apr;44(4):1435-9
pubmed: 16597873
Elife. 2020 Mar 25;9:
pubmed: 32209228
BMC Public Health. 2018 Mar 15;18(1):358
pubmed: 29544463
Lancet Microbe. 2021 Jun;2(6):e250-e258
pubmed: 35544170
Parasit Vectors. 2014 Sep 04;7:420
pubmed: 25189460
BMJ. 2019 Jan 22;364:k5224
pubmed: 30670453
Parasit Vectors. 2019 May 2;12(1):195
pubmed: 31046820
Wkly Epidemiol Rec. 2016 Jun 3;91(22):287-96
pubmed: 27263128
FEMS Microbiol Lett. 2017 Oct 16;364(19):
pubmed: 28957457
N Engl J Med. 2013 Feb 7;368(6):524-32
pubmed: 23388004
Am J Trop Med Hyg. 2016 Mar;94(3):489-93
pubmed: 26787156
Acta Trop. 2021 Mar;215:105791
pubmed: 33310076
Interdiscip Perspect Infect Dis. 2014;2014:607287
pubmed: 25104958
Exp Parasitol. 2016 Aug;167:1-6
pubmed: 27119738
Parasit Vectors. 2020 Apr 21;13(1):174
pubmed: 32312301
PLoS One. 2012;7(5):e35671
pubmed: 22693548
Parasitol Res. 2008 Oct;103(5):1159-62
pubmed: 18651180
Sci Rep. 2017 Oct 17;7(1):13325
pubmed: 29042642
Kathmandu Univ Med J (KUMJ). 2019 Jan.-Mar;17(65):77-79
pubmed: 31734685
Infect Genet Evol. 2018 Nov;65:80-90
pubmed: 30016714
PLoS Pathog. 2014 Jul 03;10(7):e1004244
pubmed: 24992200
Emerg Infect Dis. 2020 Aug;26(8):1864-1869
pubmed: 32687048
Parasit Vectors. 2014 Oct 02;7:458
pubmed: 25274259
Bull World Health Organ. 1998;76(5):485-90
pubmed: 9868839
Elife. 2016 Mar 22;5:
pubmed: 27003289
Indian J Dermatol Venereol Leprol. 2020 Sep-Oct;86(5):536-539
pubmed: 32769303