Development of the isothermal recombinase polymerase amplification assays for rapid detection of the genus Capripoxvirus.
Capripoxvirus
Isothermal amplification
LFS RPA
P32 gene
Real-time RPA
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
18
04
2023
revised:
05
07
2023
accepted:
27
07
2023
medline:
22
8
2023
pubmed:
31
7
2023
entrez:
30
7
2023
Statut:
ppublish
Résumé
Sheeppox virus (SPPV), goatpox virus (GTPV) and lumpy skin disease virus (LSDV) belong to the genus Capripoxvirus (CaPV), and are important pathogens of sheep, goat and cattle, respectively. Rapid and reliable detection of CaPV is critical to prevent its spread and promote its eradication. This study aimed to develop the recombinase polymerase amplification (RPA) assays combined with real-time fluorescence (real-time RPA) and naked-eye visible lateral flow strip (LFS RPA) for rapid detection of CaPV. Both developed RPA assays worked well at 39 °C within 20 min. They were highly specific for the detection of GTPV, SPPV and LSDV, while no cross-reactivity was observed for other non-targeted pathogens and genomic DNA of goat, sheep and cattle. The limit of detection for real-time RPA and LFS RPA were 1.0 × 10
Identifiants
pubmed: 37517457
pii: S0166-0934(23)00113-1
doi: 10.1016/j.jviromet.2023.114788
pii:
doi:
Substances chimiques
Recombinases
0
Viral Proteins
0
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114788Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interests.