Targeted metagenomics using bait-capture to detect antibiotic resistance genes in retail meat and seafood.
antimicrobial resistance
beef
chicken
gene detection
oysters
resistome
shrimp
veal
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
17
03
2023
accepted:
28
06
2023
medline:
31
7
2023
pubmed:
31
7
2023
entrez:
31
7
2023
Statut:
epublish
Résumé
Metagenomics analysis of foods has the potential to provide comprehensive data on the presence and prevalence of antimicrobial resistance (AMR) genes in the microbiome of foods. However, AMR genes are generally present in low abundance compared to other bacterial genes in the food microbiome and consequently require multiple rounds of in-depth sequencing for detection. Here, a metagenomics approach, using bait-capture probes targeting antimicrobial resistance and plasmid genes, is used to characterize the resistome and plasmidome of retail beef, chicken, oyster, shrimp, and veal enrichment cultures (
Identifiants
pubmed: 37520363
doi: 10.3389/fmicb.2023.1188872
pmc: PMC10373929
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1188872Informations de copyright
Copyright © 2023 Flint, Cooper, Rao, Weedmark, Carrillo and Tamber.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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