Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants.


Journal

Environmental science & technology
ISSN: 1520-5851
Titre abrégé: Environ Sci Technol
Pays: United States
ID NLM: 0213155

Informations de publication

Date de publication:
15 08 2023
Historique:
medline: 16 8 2023
pubmed: 31 7 2023
entrez: 31 7 2023
Statut: ppublish

Résumé

Airborne bacteria and endotoxin may affect asthma and allergies. However, there is limited understanding of the environmental determinants that influence them. This study investigated the airborne microbiomes in the homes of 1038 participants from five cities in Northern Europe: Aarhus, Bergen, Reykjavik, Tartu, and Uppsala. Airborne dust particles were sampled with electrostatic dust fall collectors (EDCs) from the participants' bedrooms. The dust washed from the EDCs' clothes was used to extract DNA and endotoxin. The DNA extracts were used for quantitative polymerase chain (qPCR) measurement and 16S rRNA gene sequencing, while endotoxin was measured using the kinetic chromogenic limulus amoebocyte lysate (LAL) assay. The results showed that households in Tartu and Aarhus had a higher bacterial load and diversity than those in Bergen and Reykjavik, possibly due to elevated concentrations of outdoor bacterial taxa associated with low precipitation and high wind speeds. Bergen-Tartu had the highest difference (ANOSIM

Identifiants

pubmed: 37523308
doi: 10.1021/acs.est.3c01616
pmc: PMC10433529
doi:

Substances chimiques

Endotoxins 0
RNA, Ribosomal, 16S 0
Dust 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11750-11766

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Auteurs

Hesham Amin (H)

Department of Clinical Science, University of Bergen, 5021 Bergen, Norway.

Tina Šantl-Temkiv (T)

Section for Microbiology, Department of Biology, Aarhus University, 8000 Aarhus, Denmark.

Christine Cramer (C)

Department of Public Health, Environment, Work and Health, Danish Ramazzini Center, Aarhus University, 8000 Aarhus, Denmark.
Department of Occupational Medicine, Danish Ramazzini Center, Aarhus University Hospital, 8200 Aarhus, Denmark.

Kai Finster (K)

Section for Microbiology, Department of Biology, Aarhus University, 8000 Aarhus, Denmark.

Francisco Gomez Real (FG)

Department of Clinical Science, University of Bergen, 5021 Bergen, Norway.

Thorarinn Gislason (T)

Faculty of Medicine, University of Iceland, 102 Reykjavík, Iceland.

Mathias Holm (M)

Department of Occupational and Environmental Medicine, University of Gothenburg, 405 30 Gothenburg, Sweden.

Christer Janson (C)

Department of Medical Sciences: Respiratory, Allergy, Sleep Research, Uppsala University, 751 85 Uppsala, Sweden.
Department of Medical Sciences: Clinical Physiology, Uppsala University, 751 85 Uppsala, Sweden.

Nils Oskar Jögi (NO)

Department of Clinical Science, University of Bergen, 5021 Bergen, Norway.

Rain Jogi (R)

Tartu University Hospital, Lung Clinic, 50406 Tartu, Estonia.

Andrei Malinovschi (A)

Department of Medical Sciences: Clinical Physiology, Uppsala University, 751 85 Uppsala, Sweden.

Ian P G Marshall (IPG)

Section for Microbiology, Department of Biology, Aarhus University, 8000 Aarhus, Denmark.

Lars Modig (L)

Division of Occupational and Environmental Medicine, Department of Public Health and Clinical Medicine, Umeå University, 901 87 Umeå, Sweden.

Dan Norbäck (D)

Department of Medical Sciences, Occupational and Environmental Medicine, Uppsala University, 751 85 Uppsala, Sweden.

Rajesh Shigdel (R)

Department of Clinical Science, University of Bergen, 5021 Bergen, Norway.

Torben Sigsgaard (T)

Department of Public Health, Environment, Work and Health, Danish Ramazzini Center, Aarhus University, 8000 Aarhus, Denmark.

Cecilie Svanes (C)

Department of Occupational Medicine, Haukeland University Hospital, 5053 Bergen, Norway.
Centre for International Health, University of Bergen Department of Global Public Health and Primary Care, 5009 Bergen, Norway.

Hulda Thorarinsdottir (H)

Department of Anesthesia and Intensive Care, Landspitali University Hospital, 101 Reykjavik, Iceland.

Inge M Wouters (IM)

Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CS Utrecht, The Netherlands.

Vivi Schlünssen (V)

Department of Public Health, Environment, Work and Health, Danish Ramazzini Center, Aarhus University, 8000 Aarhus, Denmark.

Randi J Bertelsen (RJ)

Department of Clinical Science, University of Bergen, 5021 Bergen, Norway.

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Classifications MeSH