An Immunohistochemical Study of Proliferation of Human Fetal Heart Cardiomyocyte With Phospho-Histone H3 Antibody.
cardiomyocyte
fetal
human
phh3 index
proliferation
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
accepted:
29
06
2023
medline:
1
8
2023
pubmed:
1
8
2023
entrez:
1
8
2023
Statut:
epublish
Résumé
The rapid proliferation of cardiomyocytes in mammals occurs during fetal life. But in postnatal life, this capacity of proliferation is reduced or lost as they exit the cell cycle. However, the cardiomyocytes don't show the same activity for different species. In human fetuses or in adult life, the capacity of the proliferation of cardiomyocytes and their response to an injury are not understood yet. In this study, we have done an immunohistochemical study using phospho-histone H3 (PHH3) to observe human fetal cardiomyocytes' proliferative activity. The heart specimens from the fetal autopsy of spontaneously aborted and stillborn human fetuses were subjected to immunohistochemical study using PHH3 antibody, and comparison between the PHH3 index (number of PHH3 positive cells per 1000 number of cardiomyocytes/high power field [HPF]) of myocardial regions was done using appropriate statistical tests. A total of 17 fetal hearts were included in our study. In the left ventricle, right ventricle, right atrium, and interventricular septum, the PHH3 index of myocardium was significantly higher over the pericardial region (p-value 0.002, p-value <0.001, <0.001, and 0.009 respectively) as compared to the region of over the endocardium and the middle part of the myocardium. The PHH3 index of the pericardial region of the left ventricle was significantly correlated with the maximum thickness of the left ventricle.
Identifiants
pubmed: 37525760
doi: 10.7759/cureus.41159
pmc: PMC10387164
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e41159Informations de copyright
Copyright © 2023, Kar et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cell. 2018 Mar 22;173(1):104-116.e12
pubmed: 29502971
Eur J Echocardiogr. 2009 Dec;10(8):iii3-7
pubmed: 19889656
Clin Sci (Lond). 2019 Jun 7;133(11):1229-1253
pubmed: 31175264
Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1446-51
pubmed: 23302686
Acta Biomater. 2014 Jan;10(1):194-204
pubmed: 24012606
Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18455-60
pubmed: 16352730
Fetal Diagn Ther. 2020;47(5):387-398
pubmed: 30612128
Anat Rec A Discov Mol Cell Evol Biol. 2004 Jan;276(1):43-57
pubmed: 14699633
Early Hum Dev. 1997 May 28;48(3):249-59
pubmed: 9154416
Curr Cardiol Rep. 2017 Feb;19(2):13
pubmed: 28185170
Cell Mol Life Sci. 2007 Mar;64(6):692-703
pubmed: 17380310
Mol Cell Endocrinol. 2013 May 22;371(1-2):182-8
pubmed: 23376610
Cold Spring Harb Symp Quant Biol. 2002;67:45-8
pubmed: 12858522
Cell. 2015 Jun 18;161(7):1566-75
pubmed: 26073943
Science. 2011 Apr 22;332(6028):458-61
pubmed: 21512031
J Cardiovasc Electrophysiol. 1999 Nov;10(11):1525-33
pubmed: 10571372
Oncotarget. 2017 May 10;8(39):65064-65076
pubmed: 29029412
Sci Rep. 2014 May 30;4:5122
pubmed: 24874299
Appl Immunohistochem Mol Morphol. 2018 Oct;26(9):627-631
pubmed: 28777144
Circ Res. 1998 Jul 13;83(1):1-14
pubmed: 9670913
Pharmacol Res. 2018 Jan;127:129-140
pubmed: 28751220
J Mol Cell Cardiol. 2013 Sep;62:203-13
pubmed: 23751911
Mod Pathol. 2014 Sep;27(9):1246-54
pubmed: 24434900
Genes Dev. 1994 May 1;8(9):1007-18
pubmed: 7926783
Int J Gynecol Pathol. 2009 Jul;28(4):316-21
pubmed: 19483635
Stem Cell Res Ther. 2016 Nov 29;7(1):174
pubmed: 27899163
Cells Tissues Organs. 2011;194(5):349-62
pubmed: 21389672
Physiol Rev. 2007 Apr;87(2):521-44
pubmed: 17429040
Am J Surg Pathol. 2006 May;30(5):657-64
pubmed: 16699322