Evidence for RA-dependent meiosis onset in a turtle embryo.
Aldh1a1
Cyp26a1
Meiosis onset
Red-eared slider turtle
Retinoic acid
Journal
Cell and tissue research
ISSN: 1432-0878
Titre abrégé: Cell Tissue Res
Pays: Germany
ID NLM: 0417625
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
22
02
2023
accepted:
12
07
2023
pubmed:
1
8
2023
medline:
1
8
2023
entrez:
1
8
2023
Statut:
ppublish
Résumé
Meiotic entry is one of the earliest sex determination events of the germ cell in higher vertebrates. Although advances in meiosis onset have been achieved in mammals, birds and fish, how this process functions in reptiles is largely unknown. In this study, we present the molecular analysis of meiosis onset and the role of retinoic acid (RA) in this process in the red-eared slider turtle. Our results using Stra8 as a pre-meiosis indicator show that in the female embryonic gonad, meiosis commitment starts around stage 19. Additionally, signals of the meiosis marker Sycp3 could be detected at stage 19 and become highly expressed by stage 23. No expression of these genes was detected in male embryonic gonads, suggesting the entry into meiosis prophase I was restricted to female embryonic germ cells. Notably, RA activity in fetal gonads is likely to be elevated in females than that in males, as evidenced by the higher expression of RA synthase Aldh1a1 and lower expression of RA-degrading enzyme Cyp26a1 in female gonads prior to meiotic entry. In addition, exogenous RA treatment induced the expression of Stra8 and Sycp3 in both sexes, whether in vivo or in vitro. Together, these results indicate that high levels of RA in the embryonic female gonads can lead to the initiation of meiosis in the turtle.
Identifiants
pubmed: 37526735
doi: 10.1007/s00441-023-03814-1
pii: 10.1007/s00441-023-03814-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
229-241Subventions
Organisme : National Natural Science Foundation of China
ID : 31922084
Organisme : National Natural Science Foundation of China
ID : 31872960
Organisme : National Natural Science Foundation of China
ID : U22A20529
Organisme : Science Fund for Distinguished Young Scholars of Zhejiang Province
ID : LR19C190001
Organisme : Postdoctoral Research Foundation of China
ID : 2022M722982
Organisme : Natural Science Foundation of Ningbo
ID : 2022J192
Organisme : Zhejiang Provincial Top Key Discipline of Biological Engineering
ID : 1741000592
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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