TMEM2 is a bona fide hyaluronidase possessing intrinsic catalytic activity.
HYAL2
TMEM2
enzyme
enzyme purification
hyaluronan
hyaluronidase
recombinant protein expression
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
24
06
2023
revised:
25
07
2023
accepted:
27
07
2023
pubmed:
2
8
2023
medline:
2
8
2023
entrez:
1
8
2023
Statut:
ppublish
Résumé
Transmembrane protein 2 (TMEM2) was originally identified as a membrane-anchored protein of unknown function. We previously demonstrated that TMEM2 can degrade hyaluronan (HA). Furthermore, we showed that induced global knockout of Tmem2 in adult mice results in rapid accumulation of incompletely degraded HA in bodily fluids and organs, supporting the identity of TMEM2 as a cell surface hyaluronidase. In spite of these advances, no direct evidence has been presented to demonstrate the intrinsic hyaluronidase activity of TMEM2. Here, we directly establish the catalytic activity of TMEM2. The ectodomain of TMEM2 (TMEM2
Identifiants
pubmed: 37527776
pii: S0021-9258(23)02148-8
doi: 10.1016/j.jbc.2023.105120
pmc: PMC10474455
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105120Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.