Characterization of chemical ingredients and in rats metabolic profiling of Lingyang Qingfei pills via ultra-high-performance liquid chromatography combined with Quadrupole-Exactive Orbitrap high-resolution mass spectrometry.
Lingyang Qingfei pills
bio-samples
chemical ingredients
mass spectrometry
metabolites
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
07
07
2023
received:
03
10
2022
accepted:
18
07
2023
pubmed:
2
8
2023
medline:
2
8
2023
entrez:
1
8
2023
Statut:
ppublish
Résumé
Lingyang Qingfei pills (LQP), the renowned traditional Chinese medicine recipe, have been extensively utilized for the therapy of xerostomia, sore throat, bronchiolitis, and pneumonia in clinics. However, its phytochemicals remain equivocal, which severely limits the development of quality control and activity mechanisms. In the current research, a trusted method founded on ultra-high performance liquid chromatography with Quadrupole-Exactive Orbitrap mass spectrometry technique was proposed for the comprehensive screening of in vitro and in vivo chemical compositions of LQP. As a consequence, 239 constituents were preliminarily characterized, 37 of which were accurately confirmed by reference standards. In addition, a total of 208 xenobiotics, containing 71 absorbed prototypes and 137 metabolites, were revealed in rat plasma, bile, urine, and feces, respectively. The metabolic reaction of hydrolysis, hydroxylation, methylation, glycosylation, sulfation, and mixed-mode was detected in the biotransformations of flavonoids, terpenoids, alkaloids, anthraquinones, organic acids, phenylpropanoids, and so forth. And 12 of the metabolites were new compounds. This experiment acted as the first reference for chemical substances and metabolites of LQP, which could provide valuable chemical information for further clarifying pharmacodynamic substances and pharmacokinetic studies.
Identifiants
pubmed: 37528471
doi: 10.1002/jssc.202200793
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200793Informations de copyright
© 2023 Wiley-VCH GmbH.
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