Plant growth promoting Bacillus species elicit defense against Meloidogyne incognita infecting tomato in polyhouse.
Bacillus cereus
Bacillus megaterium
Bacillus pumilus
Bacillus subtilis
Meloidogyne incognita
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
12
06
2023
received:
28
03
2023
accepted:
01
07
2023
medline:
6
11
2023
pubmed:
2
8
2023
entrez:
1
8
2023
Statut:
ppublish
Résumé
The effects of four nematicidal rhizobacterial isolates; Bacillus subtilis, Bacillus pumilus, Bacillus megaterium, and Bacillus cereus on infection and multiplication of root-knot nematode, Meloidogyne incognita on tomato were compared with the application of a chemical nematicide, fluopyram 34.48% SC (Velum Prime). The bio-efficacy trial conducted in pots preinoculated with the above isolates followed by M. incognita inoculation resulted in a significant reduction in percent root galling viz. 91.95 in B. subtilis, 84.21 in B. pumilus, 83.70 in B. megaterium, and 81.8 in B. cereus, at 75 days after inoculation (DAI). The reproduction factor of the nematode was the lowest (15.83) in B. subtilis, followed by B. pumilus (21.00), compared with 48.16 in control, with enhanced photosynthetic and transpiration rates. The mechanism of induced resistance was assessed using quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantification of three key defense genes (PR-1b, JERF3, and CAT) at 0,2,4,8 and16 days DAI. The defence genes, PR-1b, JERF3, and CAT were expressed at 2.5-7.5-folds in rhizobacterialtreated plants, but not in nematicide treatment. The defense enzymes viz., super oxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (PO), and phenylalanine ammonia lyase (PAL) when quantified (μmol/mg protein) showed an increase from 1.5 to 17.5 for SOD, 2.1 to 7.8 in PPO, 1.8 to 10.2 in PO, and 1.8 to 8.7 in PAL during 0 to 16 DAI, in rhizobacteria-treated plants.
Identifiants
pubmed: 37528495
doi: 10.1002/jobm.202300146
doi:
Substances chimiques
Peroxidases
EC 1.11.1.-
Catechol Oxidase
EC 1.10.3.1
Phenylalanine Ammonia-Lyase
EC 4.3.1.24
Superoxide Dismutase
EC 1.15.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1233-1241Subventions
Organisme : fellowship granted by Post Graduate School, ICAR-IARI, Pusa, New Delhi
Informations de copyright
© 2023 Wiley-VCH GmbH.
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