Extensive intragenomic variation in the internal transcribed spacer region of fungi.
Evolutionary biology
Microbiology
Molecular biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
18 Aug 2023
18 Aug 2023
Historique:
received:
15
05
2023
revised:
08
06
2023
accepted:
04
07
2023
medline:
2
8
2023
pubmed:
2
8
2023
entrez:
2
8
2023
Statut:
epublish
Résumé
Fungi are among the most biodiverse organisms in the world. Accurate species identification is imperative for studies on fungal ecology and evolution. The internal transcribed spacer (ITS) rDNA region has been widely accepted as the universal barcode for fungi. However, several recent studies have uncovered intragenomic sequence variation within the ITS in multiple fungal species. Here, we mined the genome of 2414 fungal species to determine the prevalence of intragenomic variation and found that the genomes of 641 species, about one-quarter of the 2414 species examined, contained multiple ITS copies. Of those 641 species, 419 (∼65%) contained variation among copies revealing that intragenomic variation is common in fungi. We proceeded to show how these copies could result in the erroneous description of hundreds of fungal species and skew studies evaluating environmental DNA (eDNA) especially when making diversity estimates. Additionally, many genomes were found to be contaminated, especially those of unculturable fungi.
Identifiants
pubmed: 37529098
doi: 10.1016/j.isci.2023.107317
pii: S2589-0042(23)01394-9
pmc: PMC10387565
doi:
Banques de données
Dryad
['10.5061/dryad.g79cnp5t7']
Types de publication
Journal Article
Langues
eng
Pagination
107317Subventions
Organisme : NIAID NIH HHS
ID : R01 AI153356
Pays : United States
Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no conflict of interests.
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