Evaluation of porcine intestinal organoids as an
Mammalian orthoreovirus 3
in vitro techniques, jejunum
organoid
Journal
Journal of veterinary science
ISSN: 1976-555X
Titre abrégé: J Vet Sci
Pays: Korea (South)
ID NLM: 100964185
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
16
01
2023
revised:
09
06
2023
accepted:
16
06
2023
medline:
4
8
2023
pubmed:
3
8
2023
entrez:
2
8
2023
Statut:
ppublish
Résumé
Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied. This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. We suggest that PIOs can be an
Sections du résumé
BACKGROUND
BACKGROUND
Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied.
OBJECTIVES
OBJECTIVE
This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an
METHODS
METHODS
PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as
RESULTS
RESULTS
The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner.
CONCLUSIONS
CONCLUSIONS
We suggest that PIOs can be an
Identifiants
pubmed: 37532298
pii: 24.e53
doi: 10.4142/jvs.23017
pmc: PMC10404702
doi:
Substances chimiques
Antiviral Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e53Subventions
Organisme : Ministry of Agriculture, Food and Rural Affairs
ID : N-1543083-2023-32-01
Pays : Korea
Informations de copyright
© 2023 The Korean Society of Veterinary Science.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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