Microfluidic intestinal organoid-on-a-chip uncovers therapeutic targets by recapitulating oxygen dynamics of intestinal IR injury.
Ionic barrier of ZIF-8
Microfluidics
Olfm4
Organoid-on-a-chip
Oxygen dynamics
Journal
Bioactive materials
ISSN: 2452-199X
Titre abrégé: Bioact Mater
Pays: China
ID NLM: 101685294
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
07
04
2023
revised:
11
06
2023
accepted:
01
07
2023
medline:
3
8
2023
pubmed:
3
8
2023
entrez:
3
8
2023
Statut:
epublish
Résumé
Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns. It takes ∼24 h for tri-gas incubator to achieve steady cell hypoxia, which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion (IR) injury. Inspired from the structure of native intestinal villi, we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on co-axial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold. The chip was featured on: (i) eight times the oxygen exchange efficiency compared with the conventional device, tri-gas incubator, (ii) implantation of intestinal organoid reproducing all types of intestinal epithelial cells, and (iii) bio-responsiveness to hypoxia and reoxygenation (HR) by presenting metabolism disorder, inflammatory reaction, and cell apoptosis. Strikingly, it was found for the first time that Olfactomedin 4 (
Identifiants
pubmed: 37534235
doi: 10.1016/j.bioactmat.2023.07.001
pii: S2452-199X(23)00206-2
pmc: PMC10391666
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1-14Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they do not have any competing interests.
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