Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics.
ATP
Actin
Bioenergetics
Cytoskeleton
DRG
Growth cones
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
15 08 2023
15 08 2023
Historique:
received:
23
05
2023
accepted:
26
07
2023
pmc-release:
18
08
2024
medline:
21
8
2023
pubmed:
3
8
2023
entrez:
3
8
2023
Statut:
ppublish
Résumé
The regulation of the intracellular level of ATP is a fundamental aspect of bioenergetics. Actin cytoskeletal dynamics have been reported to be an energetic drain in developing neurons and platelets. We addressed the role of actin dynamics in primary embryonic chicken neurons using luciferase assays, and by measurement of the ATP/ADP ratio using the ratiometric reporter PercevalHR and the ATP level using the ratiometric reporter mRuby-iATPSnFR. None of the methods revealed an effect of suppressing actin dynamics on the decline in the neuronal ATP level or the ATP/ADP ratio following shutdown of ATP production. Similarly, we find that treatments that elevate or suppress actin dynamics do not alter the ATP/ADP ratio in growth cones, the subcellular domain with the highest actin dynamics in developing neurons. Collectively, the data indicate that actin cytoskeletal dynamics are not a significant energy drain in developing neurons and that the ATP/ADP ratio is maintained when energy utilization varies. Discrepancies between prior work and the current data are discussed with emphasis on methodology and interpretation of the data.
Identifiants
pubmed: 37534394
pii: 325768
doi: 10.1242/jcs.261356
pmc: PMC10445737
pii:
doi:
Substances chimiques
Actins
0
Adenosine Triphosphate
8L70Q75FXE
Banques de données
figshare
['10.6084/m9.figshare.23696910.v1']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R21 NS118000
Pays : United States
Organisme : NINDS NIH HHS
ID : R56 NS128049
Pays : United States
Informations de copyright
© 2023. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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