Genetic diversity and natural selection of Plasmodium vivax reticulocyte invasion genes in Ecuador.
Ecuador
Genetic diversity
Merozoite invasion
Natural selection
Plasmodium vivax
Reticulocytes
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
03 Aug 2023
03 Aug 2023
Historique:
received:
25
01
2023
accepted:
04
07
2023
medline:
7
8
2023
pubmed:
4
8
2023
entrez:
3
8
2023
Statut:
epublish
Résumé
Knowledge of the diversity of invasion ligands in malaria parasites in endemic regions is essential to understand how natural selection influences genetic diversity of these ligands and their feasibility as possible targets for future vaccine development. In this study the diversity of four genes for merozoite invasion ligands was studied in Ecuadorian isolates of Plasmodium vivax. Eighty-eight samples from P. vivax infected individuals from the Coast and Amazon region of Ecuador were obtained between 2012 and 2015. The merozoite invasion genes pvmsp-1-19, pvdbpII, pvrbp1a-2 and pvama1 were amplified, sequenced, and compared to the Sal-1 strain. Polymorphisms were mapped and genetic relationships between haplotypes were determined. Only one nonsynonymous polymorphism was detected in pvmsp-1-19, while 44 nonsynonymous polymorphisms were detected in pvdbpII, 56 in pvrbp1a-2 and 33 in pvama1. While haplotypes appeared to be more related within each area of study and there was less relationship between parasites of the coastal and Amazon regions of the country, diversification processes were observed in the two Amazon regions. The highest haplotypic diversity for most genes occurred in the East Amazon of the country. The high diversity observed in Ecuadorian samples is closer to Brazilian and Venezuelan isolates, but lower than reported in other endemic regions. In addition, departure from neutrality was observed in Ecuadorian pvama1. Polymorphisms for pvdbpII and pvama1 were associated to B-cell epitopes. pvdbpII and pvama1 genetic diversity found in Ecuadorian P. vivax was very similar to that encountered in other malaria endemic countries with varying transmission levels and segregated by geographic region. The highest diversity of P. vivax invasion genes in Ecuador was found in the Amazonian region. Although selection appeared to have small effect on pvdbpII and pvrbp1a-2, pvama1 was influenced by significant balancing selection.
Sections du résumé
BACKGROUND
BACKGROUND
Knowledge of the diversity of invasion ligands in malaria parasites in endemic regions is essential to understand how natural selection influences genetic diversity of these ligands and their feasibility as possible targets for future vaccine development. In this study the diversity of four genes for merozoite invasion ligands was studied in Ecuadorian isolates of Plasmodium vivax.
METHODS
METHODS
Eighty-eight samples from P. vivax infected individuals from the Coast and Amazon region of Ecuador were obtained between 2012 and 2015. The merozoite invasion genes pvmsp-1-19, pvdbpII, pvrbp1a-2 and pvama1 were amplified, sequenced, and compared to the Sal-1 strain. Polymorphisms were mapped and genetic relationships between haplotypes were determined.
RESULTS
RESULTS
Only one nonsynonymous polymorphism was detected in pvmsp-1-19, while 44 nonsynonymous polymorphisms were detected in pvdbpII, 56 in pvrbp1a-2 and 33 in pvama1. While haplotypes appeared to be more related within each area of study and there was less relationship between parasites of the coastal and Amazon regions of the country, diversification processes were observed in the two Amazon regions. The highest haplotypic diversity for most genes occurred in the East Amazon of the country. The high diversity observed in Ecuadorian samples is closer to Brazilian and Venezuelan isolates, but lower than reported in other endemic regions. In addition, departure from neutrality was observed in Ecuadorian pvama1. Polymorphisms for pvdbpII and pvama1 were associated to B-cell epitopes.
CONCLUSIONS
CONCLUSIONS
pvdbpII and pvama1 genetic diversity found in Ecuadorian P. vivax was very similar to that encountered in other malaria endemic countries with varying transmission levels and segregated by geographic region. The highest diversity of P. vivax invasion genes in Ecuador was found in the Amazonian region. Although selection appeared to have small effect on pvdbpII and pvrbp1a-2, pvama1 was influenced by significant balancing selection.
Identifiants
pubmed: 37537581
doi: 10.1186/s12936-023-04640-0
pii: 10.1186/s12936-023-04640-0
pmc: PMC10398936
doi:
Substances chimiques
Antigens, Protozoan
0
Protozoan Proteins
0
Ligands
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
225Subventions
Organisme : Pontificia Universidad Católica del Ecuador
ID : M131416 and N131416
Organisme : Pontificia Universidad Católica del Ecuador
ID : M131416 and N131416
Organisme : The World Academy of Sciences
ID : 16-158 RG/BIO/LD
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
Références
Exp Parasitol. 2007 Jul;116(3):252-6
pubmed: 17336299
Proc Natl Acad Sci U S A. 2016 May 31;113(22):6277-82
pubmed: 27194724
Am J Trop Med Hyg. 2005 Sep;73(3):626-33
pubmed: 16172494
Mol Med. 1999 Jul;5(7):459-70
pubmed: 10449807
J Infect Dis. 2017 May 15;215(10):1558-1568
pubmed: 28379500
Nature. 2006 Feb 9;439(7077):741-4
pubmed: 16372020
Acta Trop. 2013 Jan;125(1):67-74
pubmed: 23031445
J Infect Dis. 2002 Aug 15;186(4):531-9
pubmed: 12195381
Sci Rep. 2018 Jul 12;8(1):10511
pubmed: 30002416
Am J Trop Med Hyg. 2010 Dec;83(6):1230-7
pubmed: 21118926
Mol Biochem Parasitol. 2009 Jun;165(2):111-21
pubmed: 19428658
PLoS Pathog. 2011 Feb 10;7(2):e1001276
pubmed: 21347343
PLoS One. 2008;3(10):e3366
pubmed: 18846221
Bioinformatics. 2009 Jun 1;25(11):1451-2
pubmed: 19346325
Mol Biochem Parasitol. 1996 Oct 1;80(2):159-69
pubmed: 8892293
Parasite Immunol. 1999 Mar;21(3):133-9
pubmed: 10205793
Mol Biochem Parasitol. 2001 Jan 15;112(1):91-101
pubmed: 11166390
Science. 2005 Apr 15;308(5720):408-11
pubmed: 15731407
Am J Trop Med Hyg. 2009 Jan;80(1):112-8
pubmed: 19141848
Vaccine. 2010 Aug 31;28(38):6183-90
pubmed: 20654667
Malar J. 2015 Nov 16;14:455
pubmed: 26572984
Malar J. 2010 Jan 21;9:29
pubmed: 20092651
J Infect Dis. 2004 Nov 1;190(9):1556-62
pubmed: 15478059
Mol Biochem Parasitol. 1996 Jun;78(1-2):269-72
pubmed: 8813697
Mol Cell Proteomics. 2006 Jul;5(7):1286-99
pubmed: 16603573
Mol Biochem Parasitol. 1992 Feb;50(2):307-15
pubmed: 1741018
Malar J. 2008 Jun 26;7:112
pubmed: 18582360
Mol Biochem Parasitol. 2000 Dec;111(2):253-60
pubmed: 11163434
Mol Biochem Parasitol. 2007 Jan;151(1):59-69
pubmed: 17097159
Malar J. 2010 Nov 22;9:334
pubmed: 21092207
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16348-53
pubmed: 12466500
J Immunol. 2019 May 1;202(9):2648-2660
pubmed: 30944159
Methods Mol Biol. 1996;50:263-91
pubmed: 8751365
Nat Microbiol. 2019 Sep;4(9):1486-1496
pubmed: 31133752
Trends Parasitol. 2008 Jan;24(1):29-34
pubmed: 18023618
PLoS Pathog. 2015 Feb 27;11(2):e1004670
pubmed: 25723550
Infect Immun. 2004 Mar;72(3):1557-67
pubmed: 14977962
Infect Immun. 2004 Oct;72(10):5775-82
pubmed: 15385477
PLoS One. 2011;6(8):e22944
pubmed: 21829672
Cell. 1990 Oct 5;63(1):141-53
pubmed: 2170017
Malar J. 2014 Jun 14;13:233
pubmed: 24930015
Sci Rep. 2016 May 31;6:26993
pubmed: 27244695
J Immunol. 2004 Jul 1;173(1):666-72
pubmed: 15210830
PLoS Negl Trop Dis. 2013 Oct 31;7(10):e2506
pubmed: 24205419
J Immunol. 2008 Feb 1;180(3):1451-61
pubmed: 18209040
Trends Parasitol. 2004 Aug;20(8):388-95
pubmed: 15246323
PLoS One. 2014 Aug 22;9(8):e105828
pubmed: 25148251
Malar J. 2012 Mar 01;11:60
pubmed: 22380592
Mol Biochem Parasitol. 2001 Apr 6;113(2):279-87
pubmed: 11295182
Cell Host Microbe. 2011 Jan 20;9(1):9-20
pubmed: 21238943
Cell. 1992 Jun 26;69(7):1213-26
pubmed: 1617731
Nucleic Acids Res. 2017 Jul 3;45(W1):W24-W29
pubmed: 28472356
Vaccine. 2007 May 4;25(18):3713-21
pubmed: 17240494
Cell Microbiol. 2011 Jun;13(6):797-805
pubmed: 21535344
Infect Genet Evol. 2009 Dec;9(6):1295-300
pubmed: 19643205
PLoS One. 2009 Dec 30;4(12):e8497
pubmed: 20041125
Acta Trop. 2014 Aug;136:89-100
pubmed: 24704284
PLoS Negl Trop Dis. 2020 Jul 8;14(7):e0008471
pubmed: 32639964
PLoS One. 2011;6(5):e20192
pubmed: 21629662
Malar J. 2017 Jul 26;16(1):300
pubmed: 28747199
J Exp Med. 1990 Jul 1;172(1):379-82
pubmed: 1694225
Vaccine. 2011 Oct 6;29(43):7491-504
pubmed: 21784116
Infect Genet Evol. 2014 Jan;21:424-35
pubmed: 24384095
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7085-9
pubmed: 1496004
Microbes Infect. 2008 Oct;10(12-13):1266-73
pubmed: 18692152
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12736-41
pubmed: 16129835
Malar J. 2012 Jun 18;11:206
pubmed: 22709605
Parasitology. 2004 Apr;128(Pt 4):353-66
pubmed: 15151140
Infect Genet Evol. 2011 Aug;11(6):1327-39
pubmed: 21554998
Mol Biol Evol. 2007 Apr;24(4):939-47
pubmed: 17244598
J Infect Dis. 2018 Aug 24;218(7):1110-1118
pubmed: 29741629
PLoS One. 2008 Aug 29;3(8):e3085
pubmed: 18769730
Parasit Vectors. 2015 Dec 21;8:651
pubmed: 26691669
PLoS Med. 2007 Dec;4(12):e337
pubmed: 18092885
Acta Trop. 2013 Jun;126(3):269-79
pubmed: 23467011
Korean J Parasitol. 2001 Jun;39(2):143-50
pubmed: 11441501
Mol Biochem Parasitol. 1994 Sep;67(1):59-68
pubmed: 7838184
Mol Biochem Parasitol. 1995 Mar;70(1-2):217-9
pubmed: 7637707