Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production.

Alanine dehydrogenase Escherichia coli L-alanine Metabolic engineering

Journal

Biotechnology for biofuels and bioproducts
ISSN: 2731-3654
Titre abrégé: Biotechnol Biofuels Bioprod
Pays: England
ID NLM: 9918300888906676

Informations de publication

Date de publication:
03 Aug 2023
Historique:
received: 16 04 2023
accepted: 20 07 2023
medline: 4 8 2023
pubmed: 4 8 2023
entrez: 3 8 2023
Statut: epublish

Résumé

Alanine dehydrogenase (AlaDH) belongs to oxidoreductases, and it exists in several different bacteria species and plays a key role in microbial carbon and nitrogen metabolism, spore formation and photosynthesis. In addition, AlaDH can also be applied in biosynthesis of L-alanine from cheap carbon source, such as glucose. To achieve a better performance of L-alanine accumulation, system evaluation and comparison of different AlaDH with potential application value are essential. In this study, enzymatic properties of AlaDH from Bacillus subtilis 168 (BsAlaDH), Bacillus cereus (BcAlaDH), Mycobacterium smegmatis MC Enzyme assay, biochemical characterization and structure analysis of BsAlaDH, BcAlaDH, MsAlaDH and GsAlaDH were carried out. In addition, application potential of these four AlaDHs in L-alanine productions were explored. The strategies here can be applied for developing L-alanine producing strains with high titers.

Sections du résumé

BACKGROUND BACKGROUND
Alanine dehydrogenase (AlaDH) belongs to oxidoreductases, and it exists in several different bacteria species and plays a key role in microbial carbon and nitrogen metabolism, spore formation and photosynthesis. In addition, AlaDH can also be applied in biosynthesis of L-alanine from cheap carbon source, such as glucose.
RESULTS RESULTS
To achieve a better performance of L-alanine accumulation, system evaluation and comparison of different AlaDH with potential application value are essential. In this study, enzymatic properties of AlaDH from Bacillus subtilis 168 (BsAlaDH), Bacillus cereus (BcAlaDH), Mycobacterium smegmatis MC
CONCLUSIONS CONCLUSIONS
Enzyme assay, biochemical characterization and structure analysis of BsAlaDH, BcAlaDH, MsAlaDH and GsAlaDH were carried out. In addition, application potential of these four AlaDHs in L-alanine productions were explored. The strategies here can be applied for developing L-alanine producing strains with high titers.

Identifiants

pubmed: 37537629
doi: 10.1186/s13068-023-02373-5
pii: 10.1186/s13068-023-02373-5
pmc: PMC10401832
doi:

Types de publication

Journal Article

Langues

eng

Pagination

123

Subventions

Organisme : National Natural Science Foundation of China
ID : 32270093
Organisme : National Natural Science Foundation of China
ID : 32070111
Organisme : Key R&D Program of Shandong Province, China
ID : 2022SFGC0102
Organisme : Rizhao Science and Technology Innovation Project
ID : 2020CXZX1206
Organisme : the Science and Technology Program of the University of Jinan
ID : XKY2028
Organisme : the Higher Educational Science and Technology Program of Jinan City
ID : 2021GXRC088
Organisme : the Innovation Capability Improvement Project of technology-based medium and small enterprises in Shandong Province
ID : 2021TSGC1247

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Pengfei Gu (P)

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China. bio_gupf@ujn.edu.cn.

Qianqian Ma (Q)

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.

Shuo Zhao (S)

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.

Qiang Li (Q)

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China.

Juan Gao (J)

School of Biological Science and Technology, University of Jinan, Jinan, 250022, People's Republic of China. bio_gaoj@ujn.edu.cn.

Classifications MeSH