FOXP1 orchestrates neurogenesis in human cortical basal radial glial cells.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
19
09
2022
accepted:
21
06
2023
revised:
16
08
2023
medline:
17
8
2023
pubmed:
4
8
2023
entrez:
4
8
2023
Statut:
epublish
Résumé
During cortical development, human basal radial glial cells (bRGCs) are highly capable of sustained self-renewal and neurogenesis. Selective pressures on this cell type may have contributed to the evolution of the human neocortex, leading to an increase in cortical size. bRGCs have enriched expression for Forkhead Box P1 (FOXP1), a transcription factor implicated in neurodevelopmental disorders (NDDs) such as autism spectrum disorder. However, the cell type-specific roles of FOXP1 in bRGCs during cortical development remain unexplored. Here, we examine the requirement for FOXP1 gene expression regulation underlying the production of bRGCs using human brain organoids. We examine a developmental time point when FOXP1 expression is highest in the cortical progenitors, and the bRGCs, in particular, begin to actively produce neurons. With the loss of FOXP1, we show a reduction in the number of bRGCs, as well as reduced proliferation and differentiation of the remaining bRGCs, all of which lead to reduced numbers of excitatory cortical neurons over time. Using single-nuclei RNA sequencing and cell trajectory analysis, we uncover a role for FOXP1 in directing cortical progenitor proliferation and differentiation by regulating key signaling pathways related to neurogenesis and NDDs. Together, these results demonstrate that FOXP1 regulates human-specific features in early cortical development.
Identifiants
pubmed: 37540706
doi: 10.1371/journal.pbio.3001852
pii: PBIOLOGY-D-22-02052
pmc: PMC10431666
doi:
Substances chimiques
Forkhead Transcription Factors
0
FOXP1 protein, human
0
Repressor Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3001852Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC014702
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH103517
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH126481
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH102603
Pays : United States
Organisme : NINDS NIH HHS
ID : UF1 NS115821
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011641
Pays : United States
Informations de copyright
Copyright: © 2023 Park et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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