A novel internet sampling for HIV surveillance: feasibility of self-sampling and preparation of DBS for delivery detection of HIV total nucleic acid and complementarity to sentinel surveillance.

Male Humans HIV Infections / diagnosis Homosexuality, Male Sentinel Surveillance Nucleic Acids Feasibility Studies Sexual and Gender Minorities Internet

DBS HIV total nucleic acid Internet plus Self-sampling Sentinel surveillance

Journal

BMC infectious diseases

ISSN: 1471-2334

Titre abrégé: BMC Infect Dis

Pays: England

ID NLM: 100968551

Informations de publication

Date de publication:
04 Aug 2023

Historique:

received: 30 03 2023

accepted: 10 07 2023

medline: 7 8 2023

pubmed: 5 8 2023

entrez: 4 8 2023

Statut: epublish

Résumé

To propose a new mode of HIV test and surveillance among population of men who have sex with men (MSM): Internet-based Self-sampling at home plus Laboratory testing of HIV total nucleic acid (TNA) in dried blood spot (DBS) (ISL of DBS TNA). Feasibility of ISL of DBS TNA was studied. Characteristics of the new mode and that of conventional surveillance mode at HIV voluntary counseling and testing clinic (VCT) were compared. A non-governmental organization (NGO) published the recruitment information on the WeChat public account. MSM filled in the questionnaire online, applied for self-sampling service package, and mailed the self made DBS to professional laboratory. The laboratory performed HIV TNA test and submitted the test results to online platform. Participants queried test results online with their unique ID. Center for Disease Control and Prevention (CDC) followed up participants with positive nucleic acid results using IDs and contact information. Rates were compared by using the Chi-Square test or Fisher's exact test. Four hundred twenty-three questionnaires were completed. 423 self-sampling service packages were sent out and 340 DBSs were returned to professional laboratory within one month with qualified rate of sampling as high as 95.0% (323/340). Seven samples were found to be TNA positive. Comparing ISL of DBS TNA with sentinel surveillance, it was found that there was a significant difference in the composition ratio of the two modes of surveillance population (P < 0.05). HIV prevalence of ISL of DBS as 2.17% was significantly lower than sentinel site as 8.96% (χ ISL of DBS TNA proposed is feasible and has a high self-sampling qualification rate, good confidentiality. It is an effective supplement to routine sentinel surveillance and has important promotion value.

Sections du résumé

BACKGROUND BACKGROUND

METHODS METHODS

A non-governmental organization (NGO) published the recruitment information on the WeChat public account. MSM filled in the questionnaire online, applied for self-sampling service package, and mailed the self made DBS to professional laboratory. The laboratory performed HIV TNA test and submitted the test results to online platform. Participants queried test results online with their unique ID. Center for Disease Control and Prevention (CDC) followed up participants with positive nucleic acid results using IDs and contact information. Rates were compared by using the Chi-Square test or Fisher's exact test.

RESULTS RESULTS

Four hundred twenty-three questionnaires were completed. 423 self-sampling service packages were sent out and 340 DBSs were returned to professional laboratory within one month with qualified rate of sampling as high as 95.0% (323/340). Seven samples were found to be TNA positive. Comparing ISL of DBS TNA with sentinel surveillance, it was found that there was a significant difference in the composition ratio of the two modes of surveillance population (P < 0.05). HIV prevalence of ISL of DBS as 2.17% was significantly lower than sentinel site as 8.96% (χ

CONCLUSIONS CONCLUSIONS

ISL of DBS TNA proposed is feasible and has a high self-sampling qualification rate, good confidentiality. It is an effective supplement to routine sentinel surveillance and has important promotion value.

Identifiants

DOI: 10.1186/s12879-023-08456-w PMID: 37542229 PMC: PMC10401770

pubmed: 37542229

doi: 10.1186/s12879-023-08456-w

pii: 10.1186/s12879-023-08456-w

pmc: PMC10401770

doi:

Substances chimiques

Nucleic Acids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

509

Subventions

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101

Organisme : National Science and Technology Major Project of China During the 13th Five-year Plan Period

ID : 2017ZX10201101