The interwoven fibril-like structure of amyloid-beta plaques in mouse brain tissue visualized using super-resolution STED microscopy.
Alzheimer’s disease
Amyloid beta peptide
Amyloid fibrils
Immunohistochemistry
Next generation drug design
Super-resolution STED microscopy
Journal
Cell & bioscience
ISSN: 2045-3701
Titre abrégé: Cell Biosci
Pays: England
ID NLM: 101561195
Informations de publication
Date de publication:
04 Aug 2023
04 Aug 2023
Historique:
received:
01
05
2023
accepted:
14
07
2023
medline:
5
8
2023
pubmed:
5
8
2023
entrez:
4
8
2023
Statut:
epublish
Résumé
Standard neuropathologic analysis of Alzheimer's brain relies on traditional fluorescence microscopy, which suffers from limited spatial resolution due to light diffraction. As a result, it fails to reveal intricate details of amyloid plaques. While electron microscopy (EM) offers higher resolution, its extensive sample preparation, involving fixation, dehydration, embedding, and sectioning, can introduce artifacts and distortions in the complex brain tissue. Moreover, EM lacks molecular specificity and has limited field of view and imaging depth. In our study, we employed super-resolution Stimulated Emission Depletion (STED) microscopy in conjunction with the anti-human APP recombinant antibody 1C3 fluorescently labelled with DyLight The utilization of STED microscopy represents a groundbreaking advancement in the field, enabling researchers to delve into the characterization of local mechanisms that underlie Amyloid (Aβ) deposition into plaques and their subsequent clearance. This unprecedented level of detail is especially crucial for comprehending the etiology of Alzheimer's disease and developing the next generation of anti-amyloid treatments. By facilitating the evaluation of drug candidates and non-pharmacological interventions aiming to reduce amyloid burden, STED microscopy emerges as an indispensable tool for driving scientific progress in Alzheimer's research.
Sections du résumé
BACKGROUND
BACKGROUND
Standard neuropathologic analysis of Alzheimer's brain relies on traditional fluorescence microscopy, which suffers from limited spatial resolution due to light diffraction. As a result, it fails to reveal intricate details of amyloid plaques. While electron microscopy (EM) offers higher resolution, its extensive sample preparation, involving fixation, dehydration, embedding, and sectioning, can introduce artifacts and distortions in the complex brain tissue. Moreover, EM lacks molecular specificity and has limited field of view and imaging depth.
RESULTS
RESULTS
In our study, we employed super-resolution Stimulated Emission Depletion (STED) microscopy in conjunction with the anti-human APP recombinant antibody 1C3 fluorescently labelled with DyLight
CONCLUSIONS
CONCLUSIONS
The utilization of STED microscopy represents a groundbreaking advancement in the field, enabling researchers to delve into the characterization of local mechanisms that underlie Amyloid (Aβ) deposition into plaques and their subsequent clearance. This unprecedented level of detail is especially crucial for comprehending the etiology of Alzheimer's disease and developing the next generation of anti-amyloid treatments. By facilitating the evaluation of drug candidates and non-pharmacological interventions aiming to reduce amyloid burden, STED microscopy emerges as an indispensable tool for driving scientific progress in Alzheimer's research.
Identifiants
pubmed: 37542303
doi: 10.1186/s13578-023-01086-4
pii: 10.1186/s13578-023-01086-4
pmc: PMC10403925
doi:
Types de publication
Journal Article
Langues
eng
Pagination
142Subventions
Organisme : Vetenskapsrådet
ID : 2018-05337
Organisme : Vetenskapsrådet
ID : 2022-03402
Organisme : Stiftelsen Olle Engkvist Byggmästare
ID : 199-0480
Organisme : Magnus Bergvalls Stiftelse
ID : 2021-04376
Organisme : UAB-GE-26408
ID : UAB-GE-26408
Organisme : HORIZON EUROPE Reforming and enhancing the European Research and Innovation system
ID : No. 737390
Informations de copyright
© 2023. Society of Chinese Bioscientists in America (SCBA).
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