In vitro evaluation of crosslinked bovine pericardium as potential scaffold for the oral cavity.
Bovine pericardium
dental pulp stem cells
guided bone regeneration
oral cavity
Journal
Bio-medical materials and engineering
ISSN: 1878-3619
Titre abrégé: Biomed Mater Eng
Pays: Netherlands
ID NLM: 9104021
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
14
11
2023
pubmed:
7
8
2023
entrez:
7
8
2023
Statut:
ppublish
Résumé
Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity. To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR. BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs). The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs. This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.
Sections du résumé
BACKGROUND
BACKGROUND
Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity.
OBJECTIVE
OBJECTIVE
To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR.
METHODS
METHODS
BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs).
RESULTS
RESULTS
The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs.
CONCLUSION
CONCLUSIONS
This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.
Identifiants
pubmed: 37545206
pii: BME230027
doi: 10.3233/BME-230027
pmc: PMC10657658
doi:
Substances chimiques
Glutaral
T3C89M417N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
561-575Références
PLoS One. 2015 Jul 20;10(7):e0132344
pubmed: 26191793
J Mater Chem B. 2015 Feb 21;3(7):1415-1425
pubmed: 32264492
J Biomater Appl. 2013 Nov;28(4):552-65
pubmed: 23142967
Biomed Mater Eng. 2021;32(4):217-228
pubmed: 33780360
Eur J Oral Sci. 2017 Oct;125(5):315-337
pubmed: 28833567
J Surg Res. 2009 Mar;152(1):135-9
pubmed: 18619621
J Tissue Eng Regen Med. 2019 Oct;13(10):1805-1814
pubmed: 31291062
Ann Vasc Surg. 2011 May;25(4):561-8
pubmed: 21276709
Stem Cells Int. 2017;2017:9082869
pubmed: 28553359
Biophys J. 2011 Jul 6;101(1):228-36
pubmed: 21723833
Interact Cardiovasc Thorac Surg. 2019 Mar 08;29(2):302–311
pubmed: 30848795
Biomaterials. 2002 Dec;23(24):4811-8
pubmed: 12361620
Mater Sci Eng C Mater Biol Appl. 2013 Apr 1;33(3):1561-72
pubmed: 23827609
Methods Mol Biol. 2014;1180:31-43
pubmed: 25015141
J Biomech. 2018 Oct 26;80:166-170
pubmed: 30170838
Xenotransplantation. 2019 Mar;26(2):e12464
pubmed: 30264494
Polymers (Basel). 2022 Feb 23;14(5):
pubmed: 35267700
Int J Biol Macromol. 2005 Sep 28;36(5):299-304
pubmed: 16102806
Nature. 2001 Nov 1;414(6859):118-21
pubmed: 11689957
Acta Biomater. 2011 Mar;7(3):1241-8
pubmed: 21094703
Am J Pathol. 1986 Apr;123(1):134-45
pubmed: 2421577
J Biomed Mater Res B Appl Biomater. 2014 May;102(4):815-25
pubmed: 24142538
Clin Implant Dent Relat Res. 2019 Aug;21(4):521-530
pubmed: 30884111
J Periodontal Res. 2014 Aug;49(4):499-507
pubmed: 24024647
J Mater Sci Mater Med. 2012 Jun;23(6):1387-96
pubmed: 22454140
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5;140:356-63
pubmed: 25617846
Open Dent J. 2014 May 16;8:56-65
pubmed: 24894890
J Stomatol Oral Maxillofac Surg. 2019 Jun;120(3):216-223
pubmed: 30579853
Mater Sci Eng C Mater Biol Appl. 2013 May 1;33(4):2392-8
pubmed: 23498274
Biopolymers. 1975 May;14(5):937-57
pubmed: 1156652
Acta Biomater. 2019 Jan 15;84:208-221
pubmed: 30342283
J Mech Behav Biomed Mater. 2019 Aug;96:27-37
pubmed: 31029992
Biomaterials. 2011 Apr;32(12):3233-43
pubmed: 21296410
Polymers (Basel). 2016 Mar 29;8(4):
pubmed: 30979206
J Clin Periodontol. 2019 Jun;46 Suppl 21:82-91
pubmed: 31215114
J Biomed Mater Res. 1999 Sep 15;46(4):531-8
pubmed: 10398014
Biomaterials. 2012 Sep;33(27):6320-44
pubmed: 22695066
Biomolecules. 2020 Feb 28;10(3):
pubmed: 32121155
J Mater Sci Mater Med. 2022 Jan 24;33(2):18
pubmed: 35072812
J Tissue Eng Regen Med. 2018 Jul;12(7):1608-1620
pubmed: 29749108
J Hand Ther. 2012 Apr-Jun;25(2):123-31; quiz 132
pubmed: 22047807
Am J Pathol. 1987 Apr;127(1):122-30
pubmed: 3105321