Type VII secretion system and its effect on group B Streptococcus virulence in isolates obtained from newborns with early onset disease and colonized pregnant women.
Streptococcus agalactiae
early onset disease
neonate
pregnancy
sepsis
type 7 secretion system
virulence
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
17
02
2023
accepted:
03
07
2023
medline:
8
8
2023
pubmed:
7
8
2023
entrez:
7
8
2023
Statut:
epublish
Résumé
GBS may cause a devastating disease in newborns. In early onset disease of the newborn the bacteria are acquired from the colonized mother during delivery. We characterized type VII secretion system (T7SS), exporting small proteins of the WXG100 superfamily, in group B Streptococci (GBS) isolates from pregnant colonized women and newborns with early onset disease (EOD) to better understand T7SS contribution to virulence in these different clinical scenarios. GBS genomes [N=33, 17 EOD isolates (serotype III/ST17) and 16 colonizing isolates (12 serotype VI/ST1, one serotype VI/ST19, one serotype VI/ST6, and two serotype 3/ST19)] were analyzed for presence of T7SS genes and genes encoding WXG100 proteins. We also perform bioinformatic analysis. Most GBS T7SS loci encoded core component genes: essC, membrane-embedded proteins (essA; essB), modulators of T7SS activity (esaA; esaB; esaC) and effectors: [esxA (SAG1039); esxB (SAG1030)].Bioinformatic analysis indicated that based on sequence type (ST) the clinicalGBS isolates encode at least three distinct subtypes of T7SS machinery. In all ST1isolates we identified two copies of esxA gene (encoding putative WXG100proteins), when only 23.5% of the ST17 isolates harbored the esxA gene. Five ST17isolates encoded two copies of the essC gene. Orphaned WXG100 molecule(SAG0230), distinct from T7SS locus, were found in all tested strains, except inST17 strains where the locus was found in only 23.5% of the isolates. In ST6 andST19 isolates most of the structure T7SS genes were missing. EOD isolates demonstrated enhanced virulence in We demonstrated that T7SS plays a role during infection. Knocking out the essC gene, considered the driver of T7SS, decreased the virulence of ST17 responsible for EOD, causing them to be less virulent comparable to the virulence observed in colonizing isolates.
Identifiants
pubmed: 37545859
doi: 10.3389/fcimb.2023.1168530
pmc: PMC10400891
doi:
Substances chimiques
Type VII Secretion Systems
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1168530Informations de copyright
Copyright © 2023 Schindler, Rahav, Nissan, Valenci, Ravins, Hanski, Ment, Tekes-Manova and Maor.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nucleic Acids Res. 2017 Jan 4;45(D1):D535-D542
pubmed: 27899627
PLoS Pathog. 2021 Jan 6;17(1):e1009182
pubmed: 33406160
Tuberculosis (Edinb). 2015 Jun;95 Suppl 1:S150-4
pubmed: 25732627
Nat Rev Microbiol. 2021 Sep;19(9):567-584
pubmed: 34040228
Virulence. 2019 Dec;10(1):600-609
pubmed: 31230520
Mol Microbiol. 2021 Mar;115(3):478-489
pubmed: 33410158
EMBO J. 2005 Jul 20;24(14):2491-8
pubmed: 15973432
Infect Immun. 2005 Sep;73(9):6151-3
pubmed: 16113338
Mol Microbiol. 2023 Jun 26;:
pubmed: 37357823
J Bacteriol. 2008 Nov;190(21):7004-11
pubmed: 18723613
Front Microbiol. 2018 Mar 14;9:437
pubmed: 29593684
J Clin Microbiol. 2020 Dec 17;59(1):
pubmed: 33115849
PLoS One. 2014 Feb 26;9(2):e89313
pubmed: 24586681
J Bacteriol. 2008 Mar;190(6):1956-65
pubmed: 18203834
Cell. 2015 Apr 23;161(3):501-512
pubmed: 25865481
Front Microbiol. 2023 Feb 13;14:1093288
pubmed: 36860481
Virulence. 2013 Jul 1;4(5):419-28
pubmed: 23652836
J Bacteriol. 2017 Oct 31;199(23):
pubmed: 28874412
Vet Microbiol. 2017 Jun;205:26-33
pubmed: 28622857
PLoS One. 2020 Dec 31;15(12):e0244450
pubmed: 33382792
Nat Commun. 2014 Aug 04;5:4544
pubmed: 25088811
Front Cell Infect Microbiol. 2019 Oct 18;9:360
pubmed: 31681636
Benef Microbes. 2018 Feb 27;9(2):257-268
pubmed: 29124967
Trends Microbiol. 2017 Nov;25(11):919-931
pubmed: 28633864
Nature. 2019 Dec;576(7786):321-325
pubmed: 31597161
J Pediatr Nurs. 2004 Oct;19(5):357-63
pubmed: 15614260
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12391-6
pubmed: 12200547
Microbiol Spectr. 2016 Feb;4(1):
pubmed: 26999395
Virulence. 2016 Apr 2;7(3):214-29
pubmed: 26730990
Trends Microbiol. 2002 May;10(5):209-12
pubmed: 11973144
Virulence. 2019 Dec;10(1):527-541
pubmed: 31142220
Nat Microbiol. 2016 Oct 10;2:16183
pubmed: 27723728
Nat Rev Microbiol. 2015 Jun;13(6):343-59
pubmed: 25978706
Front Cell Infect Microbiol. 2022 May 19;12:880943
pubmed: 35663471
J Fungi (Basel). 2018 Dec 26;5(1):
pubmed: 30587801
PLoS Pathog. 2021 Dec 6;17(12):e1010121
pubmed: 34871327
BMC Genomics. 2016 Mar 11;17:222
pubmed: 26969225
Science. 2016 Oct 21;354(6310):347-350
pubmed: 27846571
J Invertebr Pathol. 2020 Feb;170:107327
pubmed: 31945326