Maternal
TORCH infections
Toxoplasma gondii
cell cycle proteins
congenital toxoplasmosis
cyclins
retinal neurogenesis
retinal progenitor cells
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2023
2023
Historique:
received:
24
04
2023
accepted:
29
06
2023
medline:
7
8
2023
pubmed:
7
8
2023
entrez:
7
8
2023
Statut:
epublish
Résumé
Toxoplasmosis affects one third of the world population and has the protozoan Pregnant females of pigmented C57BL/6 strain mice were infected intragastrically with two Infected embryos had significantly smaller body sizes and weights than the PBS-treated controls, indicating that embryonic development was affected. In the retina, a significant increase in the number of Ki-67-positive cells (marker of proliferating cells) was found in the apical region of the NBL of infected mice compared to the control. Supporting this, cell cycle proteins Cyclin D3, Cdk6 and pChK2 were significantly altered in infected retinas. Interestingly, the immunohistochemical analysis showed a significant increase in the population of β-III-tubulin-positive cells, one of the earliest markers of neuronal differentiation. Our data suggests that CT affects cell cycle progression in retinal progenitor cells, possibly inducing the arrest of these cells at G2/M phase. Such alterations could influence the differentiation, anticipating/increasing neuronal maturation, and therefore leading to abnormal retinal formation. Our model mimics important events observed in ocular CT.
Sections du résumé
Background
UNASSIGNED
Toxoplasmosis affects one third of the world population and has the protozoan
Methods
UNASSIGNED
Pregnant females of pigmented C57BL/6 strain mice were infected intragastrically with two
Results
UNASSIGNED
Infected embryos had significantly smaller body sizes and weights than the PBS-treated controls, indicating that embryonic development was affected. In the retina, a significant increase in the number of Ki-67-positive cells (marker of proliferating cells) was found in the apical region of the NBL of infected mice compared to the control. Supporting this, cell cycle proteins Cyclin D3, Cdk6 and pChK2 were significantly altered in infected retinas. Interestingly, the immunohistochemical analysis showed a significant increase in the population of β-III-tubulin-positive cells, one of the earliest markers of neuronal differentiation.
Conclusions
UNASSIGNED
Our data suggests that CT affects cell cycle progression in retinal progenitor cells, possibly inducing the arrest of these cells at G2/M phase. Such alterations could influence the differentiation, anticipating/increasing neuronal maturation, and therefore leading to abnormal retinal formation. Our model mimics important events observed in ocular CT.
Identifiants
pubmed: 37545879
doi: 10.3389/fncel.2023.1211446
pmc: PMC10400775
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1211446Informations de copyright
Copyright © 2023 Campos, Magalhães, da Rosa, dos Santos, Fragel-Madeira, Figueiredo, Calaza and Adesse.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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