The Role of Chemically Innocent Polyanions in Active, Chemically Fueled Complex Coacervate Droplets.

Chemically Fueled Coacervation Membraneless Organelles Peptides Polyelectrolytes Protocells

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
09 Oct 2023
Historique:
received: 01 07 2023
medline: 7 8 2023
pubmed: 7 8 2023
entrez: 7 8 2023
Statut: ppublish

Résumé

Complex coacervation describes the liquid-liquid phase separation of oppositely charged polymers. Active coacervates are droplets in which one of the electrolyte's affinity is regulated by chemical reactions. These droplets are particularly interesting because they are tightly regulated by reaction kinetics. For example, they serve as a model for membraneless organelles that are also often regulated by biochemical transformations such as post-translational modifications. They are also a great protocell model or could be used to synthesize life-they spontaneously emerge in response to reagents, compete, and decay when all nutrients have been consumed. However, the role of the unreactive building blocks, e.g., the polymeric compounds, is poorly understood. Here, we show the important role of the chemically innocent, unreactive polyanion of our chemically fueled coacervation droplets. We show that the polyanion drastically influences the resulting droplets' life cycle without influencing the chemical reaction cycle-either they are very dynamic or have a delayed dissolution. Additionally, we derive a mechanistic understanding of our observations and show how additives and rational polymer design help to create the desired coacervate emulsion life cycles.

Identifiants

pubmed: 37549224
doi: 10.1002/anie.202309318
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202309318

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : IRTG 2022
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC-2094 - 390783311
Organisme : H2020 European Research Council
ID : 852187 (Actidrops)
Organisme : Fonds der Chemischen Industrie
ID : Kekulé fellowship

Informations de copyright

© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

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Auteurs

Fabian Späth (F)

Department of Chemistry, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Anton S Maier (AS)

WACKER-Chair of Macromolecular Chemistry, Catalysis Research Center, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Michele Stasi (M)

Department of Chemistry, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Alexander M Bergmann (AM)

Department of Chemistry, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Kerstin Halama (K)

WACKER-Chair of Macromolecular Chemistry, Catalysis Research Center, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Monika Wenisch (M)

Department of Chemistry, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Bernhard Rieger (B)

WACKER-Chair of Macromolecular Chemistry, Catalysis Research Center, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Job Boekhoven (J)

Department of Chemistry, School of Natural Sciences, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Classifications MeSH