Momordicae Semen inhibits migration and induces apoptotic cell death by regulating c-Myc and CNOT2 in human pancreatic cancer cells.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 08 2023
07 08 2023
Historique:
received:
30
11
2022
accepted:
31
07
2023
medline:
9
8
2023
pubmed:
8
8
2023
entrez:
7
8
2023
Statut:
epublish
Résumé
Pancreatic cancer(PC) is less common than other cancers; however, it has a poor prognosis. Therefore, studying novel target signaling and anticancer agents is necessary. Momordicae Semen (MS), the seed of Momordica sochinensis Spreng, mainly found in South-East Asia, including China and Bangladesh, is used to treat various diseases because of its anticancer, antioxidant, anti-inflammatory, and antibacterial properties. However, the effect of the MS extract on pancreatic cancer cells remains unknown. In this study investigated whether the MS extract exerted an anti-cancer effect by regulating c-Myc through CNOT2. Cytotoxicity and proliferation were investigated using MTT and colony formation assays. The levels of apoptotic, oncogenic, and migration-associated factors were confirmed using immunoblotting and immunofluorescence. Wound closure was analyzed using a wound healing assay. The chemical composition of the MS methanol extracts was analyzed using liquid chromatography-mass spectrometry. We confirmed that the MS extract regulated apoptotic factors and attenuated the stability of c-Myc and its sensitivity to fetal bovine serum. Furthermore, the MS extract increased apoptosis by regulating c-Myc and CNOT2 expression and enhanced the sensitivity of 5-FU in pancreatic cancer. This study showed that the MS extract is a promising new drug for PC.
Identifiants
pubmed: 37550432
doi: 10.1038/s41598-023-39840-w
pii: 10.1038/s41598-023-39840-w
pmc: PMC10406802
doi:
Substances chimiques
Antineoplastic Agents
0
Plant Extracts
0
CNOT2 protein, human
0
Repressor Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12800Informations de copyright
© 2023. Springer Nature Limited.
Références
Mol Cancer Res. 2019 Sep;17(9):1815-1827
pubmed: 31164413
Front Oncol. 2017 Mar 03;7:28
pubmed: 28316954
Biomolecules. 2021 Oct 10;11(10):
pubmed: 34680125
Cancer Res. 2015 Nov 15;75(22):4863-75
pubmed: 26383165
Front Nutr. 2022 Jun 29;9:943911
pubmed: 35845802
Curr Med Chem. 2023;30(34):3905-3926
pubmed: 35975861
Molecules. 2020 May 14;25(10):
pubmed: 32422890
Chin J Cancer Res. 2015 Aug;27(4):332-48
pubmed: 26361403
Cells. 2020 Apr 16;9(4):
pubmed: 32316188
N Engl J Med. 2014 Sep 11;371(11):1039-49
pubmed: 25207767
Signal Transduct Target Ther. 2018 Feb 23;3:5
pubmed: 29527331
CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
pubmed: 19474385
Int J Mol Sci. 2019 Mar 15;20(6):
pubmed: 30875872
Cancer Cell Int. 2022 Jul 28;22(1):239
pubmed: 35902860
Chin Clin Oncol. 2017 Jun;6(3):24
pubmed: 28705001
Radiat Oncol. 2020 Jun 3;15(1):139
pubmed: 32493354
CA Cancer J Clin. 2021 May;71(3):209-249
pubmed: 33538338
Cancer Biol Ther. 2022 Dec 31;23(1):34-50
pubmed: 34978469
Int J Mol Sci. 2021 Sep 27;22(19):
pubmed: 34638751
CA Cancer J Clin. 2019 Jan;69(1):7-34
pubmed: 30620402
Microb Biotechnol. 2011 Nov;4(6):687-99
pubmed: 21375717
Cancer Discov. 2020 Apr;10(4):588-607
pubmed: 31941709
Mini Rev Med Chem. 2020;20(2):123-133
pubmed: 31648635
Cancer Lett. 2018 Jan 1;412:88-98
pubmed: 29024811
Mod Pathol. 2002 Apr;15(4):462-9
pubmed: 11950922
Phytomedicine. 2019 Mar 15;56:48-56
pubmed: 30668353
Int J Oncol. 2005 Apr;26(4):881-9
pubmed: 15753981
Cancer Res. 2014 Jun 1;74(11):2913-21
pubmed: 24840647
Front Pharmacol. 2021 Jan 13;11:565700
pubmed: 33519431
Ann Oncol. 2017 Apr 1;28(4):741-747
pubmed: 28104621
World J Gastroenterol. 2004 Feb 15;10(4):471-5
pubmed: 14966900
Biomedicines. 2021 Apr 06;9(4):
pubmed: 33917380
World J Hepatol. 2010 Jan 27;2(1):16-20
pubmed: 21160952
Molecules. 2019 Oct 31;24(21):
pubmed: 31683690