Gene expression changes in sickle cell reticulocytes and their clinical associations.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 08 2023
08 08 2023
Historique:
received:
05
05
2023
accepted:
03
08
2023
medline:
10
8
2023
pubmed:
9
8
2023
entrez:
8
8
2023
Statut:
epublish
Résumé
Transcriptional changes in compensatory erythropoiesis in sickle cell anemia (SCA) and their disease modulation are unclear. We detected 1226 differentially expressed genes in hemoglobin SS reticulocytes compared to non-anemic hemoglobin AA controls. Assessing developmental expression changes in hemoglobin AA erythroblasts for these genes suggests heightened terminal differentiation in early erythroblasts in SCA that diminishes toward the polychromatic to orthochromatic stage transition. Comparison of reticulocyte gene expression changes in SCA with that in Chuvash erythrocytosis, a non-anemic disorder of increased erythropoiesis due to constitutive activation of hypoxia inducible factors, identified 453 SCA-specific changes attributable to compensatory erythropoiesis. Peripheral blood mononuclear cells (PBMCs) in SCA contain elevated proportions of erythroid progenitors due to heightened erythropoiesis. Deconvolution analysis in PBMCs from 131 SCA patients detected 54 genes whose erythroid expression correlated with erythropoiesis efficiency, which were enriched with SCA-specific changes (OR = 2.9, P = 0.00063) and annotation keyword "ubiquitin-dependent protein catabolic process", "protein ubiquitination", and "protein polyubiquitination" (OR = 4.2, P = 7.5 × 10
Identifiants
pubmed: 37553354
doi: 10.1038/s41598-023-40039-2
pii: 10.1038/s41598-023-40039-2
pmc: PMC10409856
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12864Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL153161
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL111656
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL125005
Pays : United States
Organisme : NHLBI NIH HHS
ID : K23 HL125984
Pays : United States
Organisme : NHLBI NIH HHS
ID : P50 HL118006
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA108671
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2023. Springer Nature Limited.
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