Addition of a liver to the normothermic perfusion circuit reduces renal pro-inflammatory factors.
DCD
kidney NMP
liver-kidney NMP
normothermic machine perfusion
Journal
Artificial organs
ISSN: 1525-1594
Titre abrégé: Artif Organs
Pays: United States
ID NLM: 7802778
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
07
07
2023
received:
26
04
2023
accepted:
25
07
2023
medline:
15
11
2023
pubmed:
9
8
2023
entrez:
9
8
2023
Statut:
ppublish
Résumé
Normothermic machine perfusion (NMP) provides a novel platform to preserve isolated organs in an artificial condition. Our study aimed to explore the interaction between the liver and kidney at an ex vivo organ level by adding a liver to the kidney NMP circuit. Porcine kidney and liver obtained from abattoir were subjected to 9 h NMP after suffering 30-min warm ischemia time and 90-min cold ischemia time. The liver-kidney NMP group (n = 5) and the single-kidney NMP group (n = 5) were designed. During the NMP, perfusion parameters, blood gas analysis, and tissue samples were compared. The perfusate of both groups remained stable, and continuous urine production was observed during NMP. In the liver-kidney NMP group, the lactate level was low, while blood urea nitrogen increased and glucose levels decreased. After the NMP, the renal tissue in the liver-kidney group exhibited fewer histological changes such as tubular epithelium vacuolization, along with reduced expression of IL-6, IL-8, IL-1β, NLRP3, and GSDMD. Our results indicated that the expression of renal pro-inflammatory factors was reduced in the liver-kidney NMP system.
Sections du résumé
BACKGROUND
BACKGROUND
Normothermic machine perfusion (NMP) provides a novel platform to preserve isolated organs in an artificial condition. Our study aimed to explore the interaction between the liver and kidney at an ex vivo organ level by adding a liver to the kidney NMP circuit.
METHODS
METHODS
Porcine kidney and liver obtained from abattoir were subjected to 9 h NMP after suffering 30-min warm ischemia time and 90-min cold ischemia time. The liver-kidney NMP group (n = 5) and the single-kidney NMP group (n = 5) were designed. During the NMP, perfusion parameters, blood gas analysis, and tissue samples were compared.
RESULTS
RESULTS
The perfusate of both groups remained stable, and continuous urine production was observed during NMP. In the liver-kidney NMP group, the lactate level was low, while blood urea nitrogen increased and glucose levels decreased. After the NMP, the renal tissue in the liver-kidney group exhibited fewer histological changes such as tubular epithelium vacuolization, along with reduced expression of IL-6, IL-8, IL-1β, NLRP3, and GSDMD.
CONCLUSIONS
CONCLUSIONS
Our results indicated that the expression of renal pro-inflammatory factors was reduced in the liver-kidney NMP system.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1732-1741Subventions
Organisme : Guangdong Provincial Funds for High-end Medical Equipment
Organisme : Guangdong Provincial international Cooperation Base of Science and Technology
Organisme : Guangdong Provincial Key Laboratory Construction Projection on Organ Donation and Transplant Immunology
Organisme : National Natural Science Foundation of China
Informations de copyright
© 2023 International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.
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