Gut microbiota and their metabolite profiles following peripheral nerve xenotransplantation.
Gut microbiome diversity
Metabolite profiles
Peripheral nerve xenotransplantation
SCFA-Producing microbiota
Sulfate-reducing bacteria
microorganisms
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
09
02
2023
revised:
08
07
2023
accepted:
20
07
2023
medline:
9
8
2023
pubmed:
9
8
2023
entrez:
9
8
2023
Statut:
epublish
Résumé
Intestinal pathogens are associated with xenotransplantation tolerance and rejection. However, changes in the gut microbiota in patients who have undergone peripheral nerve xenotransplantation and their association with immune rejection have not yet been reported. We aimed to explore intestinal microbes and their metabolites at different time points after peripheral nerve transplantation to provide new insight into improving transplant tolerance. A peripheral nerve xenotransplantation model was constructed by suturing the segmented nerves of Sprague Dawley rats to those of C57 male mice using xenotransplantation nerve bridging. Fecal samples and intestinal contents were collected at three time points: before surgery (Pre group; n = 10), 1 month after transplantation (Pos1 m group; n = 10), and 3 months after transplantation (Pos3 m group; n = 10) for 16S DNA sequencing and nontargeted metabolome detection. Alpha diversity results suggested that species diversity was significantly downregulated after peripheral nerve xenotransplantation. There were six gut flora genera with significantly different expression levels after xenotransplantation: four were downregulated and two were upregulated. A comparison of the Pre vs. Pos1 m groups and the Pos1 m vs. Pos3 m groups revealed that the most significant differentially expressed Kyoto Encyclopedia of Genes and Genomes metabolite pathways were involved in phenylalanine, tyrosine, and tryptophan biosynthesis, as well as histidine metabolism. Metabolites with a strong relationship to the differentially expressed microbial flora were identified. Our study found lower gut microbiome diversity, with increased short-chain fatty acid (SCFA)-producing and sulfate-reducing bacteria at 1 month post peripheral nerve xenotransplantation, and these were decreased at 3 months post-transplantation. The identification of specific bacterial metabolites is essential for recognizing potential diagnostic markers of xenotransplantation rejection or characterizing therapeutic targets to prevent post-transplant infection.
Sections du résumé
Background
UNASSIGNED
Intestinal pathogens are associated with xenotransplantation tolerance and rejection. However, changes in the gut microbiota in patients who have undergone peripheral nerve xenotransplantation and their association with immune rejection have not yet been reported.
Objective
UNASSIGNED
We aimed to explore intestinal microbes and their metabolites at different time points after peripheral nerve transplantation to provide new insight into improving transplant tolerance.
Methods
UNASSIGNED
A peripheral nerve xenotransplantation model was constructed by suturing the segmented nerves of Sprague Dawley rats to those of C57 male mice using xenotransplantation nerve bridging. Fecal samples and intestinal contents were collected at three time points: before surgery (Pre group; n = 10), 1 month after transplantation (Pos1 m group; n = 10), and 3 months after transplantation (Pos3 m group; n = 10) for 16S DNA sequencing and nontargeted metabolome detection.
Results
UNASSIGNED
Alpha diversity results suggested that species diversity was significantly downregulated after peripheral nerve xenotransplantation. There were six gut flora genera with significantly different expression levels after xenotransplantation: four were downregulated and two were upregulated. A comparison of the Pre vs. Pos1 m groups and the Pos1 m vs. Pos3 m groups revealed that the most significant differentially expressed Kyoto Encyclopedia of Genes and Genomes metabolite pathways were involved in phenylalanine, tyrosine, and tryptophan biosynthesis, as well as histidine metabolism. Metabolites with a strong relationship to the differentially expressed microbial flora were identified.
Conclusion
UNASSIGNED
Our study found lower gut microbiome diversity, with increased short-chain fatty acid (SCFA)-producing and sulfate-reducing bacteria at 1 month post peripheral nerve xenotransplantation, and these were decreased at 3 months post-transplantation. The identification of specific bacterial metabolites is essential for recognizing potential diagnostic markers of xenotransplantation rejection or characterizing therapeutic targets to prevent post-transplant infection.
Identifiants
pubmed: 37554826
doi: 10.1016/j.heliyon.2023.e18529
pii: S2405-8440(23)05737-7
pmc: PMC10404661
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e18529Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Blood. 2018 Jun 28;131(26):2978-2986
pubmed: 29674425
Front Immunol. 2019 Jan 17;9:3179
pubmed: 30705680
Inflamm Bowel Dis. 2019 Aug 20;25(9):1450-1461
pubmed: 30918945
Transpl Int. 2017 Aug;30(8):745-753
pubmed: 28012226
Gut. 2014 May;63(5):727-35
pubmed: 23804561
J Clin Oncol. 2017 May 20;35(15):1650-1659
pubmed: 28296584
Nat Commun. 2018 Sep 3;9(1):3555
pubmed: 30177845
Blood. 2022 Dec 1;140(22):2385-2397
pubmed: 35969834
J Lipid Res. 2019 Feb;60(2):412-420
pubmed: 30573561
Nutrients. 2011 Oct;3(10):858-76
pubmed: 22254083
Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2247-52
pubmed: 24390544
Cell Mol Life Sci. 2017 Aug;74(16):2959-2977
pubmed: 28352996
Front Med. 2018 Jun;12(3):239-248
pubmed: 29524068
J Innate Immun. 2019;11(5):405-415
pubmed: 30286447
J Lipid Res. 1985 Aug;26(8):982-8
pubmed: 4045322
Chemosphere. 2021 Jan;263:128355
pubmed: 33297277
BMC Biotechnol. 2008 Apr 11;8:39
pubmed: 18405347
Gut Microbes. 2020 May 3;11(3):569-580
pubmed: 31696774
Brain Res Bull. 2020 Feb;155:67-80
pubmed: 31756421
Science. 2013 Aug 2;341(6145):569-73
pubmed: 23828891
Front Immunol. 2020 Jan 23;10:3060
pubmed: 32038617
Prog Neurobiol. 2011 Feb;93(2):204-30
pubmed: 21130136
Cell. 2016 Nov 3;167(4):1125-1136.e8
pubmed: 27814509
Lancet. 1983 May 28;1(8335):1206-9
pubmed: 6134000
Exp Neurol. 2004 Dec;190(2):347-55
pubmed: 15530874
Prog Lipid Res. 2019 Oct;76:101008
pubmed: 31626820
Biomed Pharmacother. 2017 Jun;90:229-236
pubmed: 28363168
NPJ Parkinsons Dis. 2021 Mar 10;7(1):27
pubmed: 33692356
Exp Neurol. 2010 May;223(1):102-11
pubmed: 19505459
Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12642-12647
pubmed: 31182590
J Pineal Res. 2020 Mar;68(2):e12627
pubmed: 31773776
J Transl Med. 2015 Aug 23;13:275
pubmed: 26298517
Exp Clin Transplant. 2021 Jul;19(7):708-716
pubmed: 34085920
Nat Rev Cancer. 2018 May;18(5):283-295
pubmed: 29449660
N Engl J Med. 2020 Feb 27;382(9):822-834
pubmed: 32101664
Transplantation. 2014 Oct 15;98(7):729-37
pubmed: 25093516
Mucosal Immunol. 2018 May;11(3):752-762
pubmed: 29411774
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8743-8
pubmed: 21555560
Sci Transl Med. 2017 Apr 19;9(386):
pubmed: 28424327
Clin Exp Immunol. 2017 Aug;189(2):197-210
pubmed: 28422316
Panminerva Med. 2010 Jun;52(2):111-24
pubmed: 20517195
Oncotarget. 2016 Apr 12;7(15):19355-66
pubmed: 27036035
Microb Biotechnol. 2019 Nov;12(6):1109-1125
pubmed: 31006995
Cell Immunol. 2020 May;351:104080
pubmed: 32139071
Immunity. 2008 Apr;28(4):454-67
pubmed: 18400188
Food Chem. 2021 Oct 30;360:129981
pubmed: 34020366
Curr Opin Allergy Clin Immunol. 2013 Jun;13(3):257-62
pubmed: 23549152
Proc Nutr Soc. 2021 Feb;80(1):37-49
pubmed: 32238208
Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17187-92
pubmed: 19805153
Front Immunol. 2018 Dec 11;9:2838
pubmed: 30619249