Bacterial amylases enable glycogen degradation by the vaginal microbiome.
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
08
07
2021
accepted:
11
07
2023
medline:
31
8
2023
pubmed:
11
8
2023
entrez:
10
8
2023
Statut:
ppublish
Résumé
The human vaginal microbiota is frequently dominated by lactobacilli and transition to a more diverse community of anaerobic microbes is associated with health risks. Glycogen released by lysed epithelial cells is believed to be an important nutrient source in the vagina. However, the mechanism by which vaginal bacteria metabolize glycogen is unclear, with evidence implicating both bacterial and human enzymes. Here we biochemically characterize six glycogen-degrading enzymes (GDEs), all of which are pullanases (PulA homologues), from vaginal bacteria that support the growth of amylase-deficient Lactobacillus crispatus on glycogen. We reveal variations in their pH tolerance, substrate preferences, breakdown products and susceptibility to inhibition. Analysis of vaginal microbiome datasets shows that these enzymes are expressed in all community state types. Finally, we confirm the presence and activity of bacterial and human GDEs in cervicovaginal fluid. This work establishes that bacterial GDEs can participate in the breakdown of glycogen, providing insight into metabolism that may shape the vaginal microbiota.
Identifiants
pubmed: 37563289
doi: 10.1038/s41564-023-01447-2
pii: 10.1038/s41564-023-01447-2
pmc: PMC10465358
doi:
Substances chimiques
Amylases
EC 3.2.1.-
Glycogen
9005-79-2
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1641-1652Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM136652
Pays : United States
Organisme : NIAID NIH HHS
ID : K08 AI130392
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2023. The Author(s).
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