Primary and hTERT-Transduced Mesothelioma-Associated Fibroblasts but Not Primary or hTERT-Transduced Mesothelial Cells Stimulate Growth of Human Mesothelioma Cells.
conditioned medium
hTERT
human telomerase reverse transcriptase
mesothelioma-associated fibroblasts
pleural mesothelial cells
pleural mesothelioma
tumor microenvironment
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
05 08 2023
05 08 2023
Historique:
received:
10
07
2023
revised:
02
08
2023
accepted:
03
08
2023
medline:
14
8
2023
pubmed:
11
8
2023
entrez:
11
8
2023
Statut:
epublish
Résumé
Pleural mesothelioma (PM) is an aggressive malignancy that develops in a unique tumor microenvironment (TME). However, cell models for studying the TME in PM are still limited. Here, we have generated and characterized novel human telomerase reverse transcriptase (hTERT)-transduced mesothelial cell and mesothelioma-associated fibroblast (Meso-CAF) models and investigated their impact on PM cell growth. Pleural mesothelial cells and Meso-CAFs were isolated from tissue of pneumothorax and PM patients, respectively. Stable expression of hTERT was induced by retroviral transduction. Primary and hTERT-transduced cells were compared with respect to doubling times, hTERT expression and activity levels, telomere lengths, proteomes, and the impact of conditioned media (CM) on PM cell growth. All transduced derivatives exhibited elevated hTERT expression and activity, and increased mean telomere lengths. Cell morphology remained unchanged, and the proteomes were similar to the corresponding primary cells. Of note, the CM of primary and hTERT-transduced Meso-CAFs stimulated PM cell growth to the same extent, while CM derived from mesothelial cells had no stimulating effect, irrespective of hTERT expression. In conclusion, all new hTERT-transduced cell models closely resemble their primary counterparts and, hence, represent valuable tools to investigate cellular interactions within the TME of PM.
Identifiants
pubmed: 37566084
pii: cells12152006
doi: 10.3390/cells12152006
pmc: PMC10417280
pii:
doi:
Substances chimiques
Proteome
0
Telomerase
EC 2.7.7.49
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Austrian Science Fund FWF
ID : I 3977
Pays : Austria
Références
Cytotechnology. 2004 Jun;45(1-2):33-8
pubmed: 19003241
Pleura Peritoneum. 2016 Jun 1;1(2):79-89
pubmed: 30911611
Respirology. 2002 Sep;7(3):171-91
pubmed: 12153683
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Cancer Cell Int. 2021 Oct 18;21(1):546
pubmed: 34663305
J Hematol Oncol. 2019 Aug 28;12(1):86
pubmed: 31462327
Genes (Basel). 2016 Aug 16;7(8):
pubmed: 27537914
Int J Biochem Cell Biol. 2004 Jan;36(1):9-16
pubmed: 14592528
Transl Oncol. 2012 Feb;5(1):56-65
pubmed: 22348177
Mol Carcinog. 2017 Mar;56(3):923-935
pubmed: 27585244
Biomedicines. 2023 Jan 16;11(1):
pubmed: 36672737
Oncogene. 2002 Jan 3;21(1):128-39
pubmed: 11791183
Front Bioeng Biotechnol. 2015 Aug 17;3:117
pubmed: 26347862
Breast Cancer Res. 2016 Aug 11;18(1):84
pubmed: 27515302
J Pathol. 2013 Dec;231(4):517-31
pubmed: 24114721
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
J Exp Clin Cancer Res. 2023 Jan 23;42(1):27
pubmed: 36683050
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14842-7
pubmed: 17003113
Cancers (Basel). 2021 Aug 20;13(16):
pubmed: 34439349
Biochem Biophys Res Commun. 2021 Sep 3;568:37-42
pubmed: 34175688
ESMO Open. 2020 Mar;4(Suppl 2):e000669
pubmed: 32156681
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14723-8
pubmed: 9843956
J Pers Med. 2021 Nov 15;11(11):
pubmed: 34834557
N Engl J Med. 2021 Sep 23;385(13):1207-1218
pubmed: 34551230
Thorac Surg Clin. 2020 Nov;30(4):383-393
pubmed: 33012428
Clin Cancer Res. 2020 Jul 15;26(14):3819-3830
pubmed: 32317288
J Virol. 1988 Apr;62(4):1120-4
pubmed: 2831375
Nucleic Acids Res. 2003 Jan 15;31(2):E3-3
pubmed: 12527792
Int J Mol Sci. 2022 Oct 18;23(20):
pubmed: 36293328
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3884-8
pubmed: 2987952
Am J Respir Crit Care Med. 2014 Oct 1;190(7):763-72
pubmed: 25188816
BMC Bioinformatics. 2012;13 Suppl 16:S12
pubmed: 23176165
Biol Proced Online. 2011 Jan 31;13:3
pubmed: 21369534
Br J Cancer. 2008 Jul 8;99(1):151-9
pubmed: 18594539
Nat Rev Cancer. 2020 Mar;20(3):174-186
pubmed: 31980749
Am J Physiol Lung Cell Mol Physiol. 2020 Oct 1;319(4):L652-L660
pubmed: 32726133
Electrophoresis. 2014 May;35(10):1428-38
pubmed: 24644141
Nat Protoc. 2018 Mar;13(3):530-550
pubmed: 29446774
Nat Protoc. 2016 Dec;11(12):2301-2319
pubmed: 27809316
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
EMBO J. 2014 Aug 1;33(15):1713-26
pubmed: 24986882
Cytotechnology. 2004 Jun;45(1-2):39-46
pubmed: 19003242
Front Immunol. 2022 Oct 04;13:1013506
pubmed: 36268019
J Cell Biochem. 2006 Jul 1;98(4):723-34
pubmed: 16795078
Front Oncol. 2022 Nov 17;12:1052457
pubmed: 36465383
Cancers (Basel). 2022 Aug 12;14(16):
pubmed: 36010899
J Pathol. 2023 Feb;259(2):205-219
pubmed: 36373776
Cancers (Basel). 2021 Nov 12;13(22):
pubmed: 34830817
Int J Biochem Cell Biol. 2004 Apr;36(4):621-42
pubmed: 15010328
PLoS One. 2011;6(10):e25391
pubmed: 21984916