Silencing of ATP1A1 attenuates cell membrane disruption by nanosecond electric pulses.
ATP1A1
Na,K-ATPase
Nanosecond electric pulses
nsEP
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
15 10 2023
15 10 2023
Historique:
received:
27
07
2023
revised:
03
08
2023
accepted:
04
08
2023
medline:
4
9
2023
pubmed:
11
8
2023
entrez:
11
8
2023
Statut:
ppublish
Résumé
This study explored the role of the Na/K-ATPase (NKA) in membrane permeabilization induced by nanosecond electric pulses. Using CRISPR/Cas9 and shRNA, we silenced the ATP1A1 gene, which encodes α1 NKA subunit in U937 human monocytes. Silencing reduced the rate and the cumulative uptake of YoPro-1 dye after electroporation by 300-ns, 7-10 kV/cm pulses, while ouabain, a specific NKA inhibitor, enhanced YoPro-1 entry. We conclude that the α1 subunit supports the electropermeabilized membrane state, by forming or stabilizing electropores or by hindering repair mechanisms, and this role is independent of NKA's ion pump function.
Identifiants
pubmed: 37566922
pii: S0006-291X(23)00943-9
doi: 10.1016/j.bbrc.2023.08.011
pii:
doi:
Substances chimiques
RNA, Small Interfering
0
Sodium-Potassium-Exchanging ATPase
EC 7.2.2.13
ATP1A1 protein, human
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
93-97Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.