Qualitative and quantitative studies on chemical constituents of Ling-gui-zhu-gan decoction: In vitro and in vivo.
Fourier transform ion cyclotron resonance mass spectrometry
Ling-gui-zhu-gan decoction
chemical constituents
identification
metabolite
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
29
07
2023
received:
27
06
2023
accepted:
02
08
2023
pubmed:
12
8
2023
medline:
12
8
2023
entrez:
11
8
2023
Statut:
ppublish
Résumé
Ling-gui-zhu-gan decoction has significant therapeutic effects in the treatment of diseases related to phlegm and fluid retention. In this study, we aimed to qualitatively characterize the chemical constituents of Ling-gui-zhu-gan decoction in vitro and in vivo by HPLC coupled to Fourier transform ion cyclotron resonance MS, and quantitively determine the contents of typical chemical constituents by HPLC method. As a result, a total of 75 chemical constituents were discovered including 37 flavonoids and their glycosides, 20 saponins, 9 sterols, 3 organic acids and their derivatives, 3 lactones, 2 coumarins, and 1 alcohol. Among them, 17 chemical constituents were specifically identified. Subsequently, an HPLC method was established for simultaneous determination of seven chemical constituents. Finally, a total of 40 prototype components were initially detected by HPLC-MS method in the biological samples of rats after their water extract was orally administrated. Among them, 29, 27, 12, and 32 prototype components were detected in plasma, bile, urine, and feces, respectively. Moreover, 34 metabolites, including 16 phase II metabolites, were detected for the first time. In conclusion, this study lays the foundation for the identification of chemical components in vitro and in vivo and the elucidation of the potential pharmacodynamic components of Ling-gui-zhu-gan decoction.
Identifiants
pubmed: 37568255
doi: 10.1002/jssc.202300465
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300465Subventions
Organisme : National Natural Science Foundation of China
ID : 82274077
Organisme : Natural Science Foundation of Liaoning Province
ID : 2021-MS-220
Organisme : Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University
ID : ZQN2018007
Organisme : Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University
ID : ZQN202204
Informations de copyright
© 2023 Wiley-VCH GmbH.
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