Bioorthogonal Engineered Virus-Like Nanoparticles for Efficient Gene Therapy.
Bioorthogonal chemistry
Gene therapy
Recombinant biosome
Site-specific codon mutation
Virus-like nanoparticle
Journal
Nano-micro letters
ISSN: 2150-5551
Titre abrégé: Nanomicro Lett
Pays: Germany
ID NLM: 101727940
Informations de publication
Date de publication:
12 Aug 2023
12 Aug 2023
Historique:
received:
10
04
2023
accepted:
20
06
2023
medline:
13
8
2023
pubmed:
13
8
2023
entrez:
12
8
2023
Statut:
epublish
Résumé
Gene therapy offers potentially transformative strategies for major human diseases. However, one of the key challenges in gene therapy is developing an effective strategy that could deliver genes into the specific tissue. Here, we report a novel virus-like nanoparticle, the bioorthgonal engineered virus-like recombinant biosome (reBiosome), for efficient gene therapies of cancer and inflammatory diseases. The mutant virus-like biosome (mBiosome) is first prepared by site-specific codon mutation for displaying 4-azido-L-phenylalanine on vesicular stomatitis virus glycoprotein of eBiosome at a rational site, and the reBiosome is then prepared by clicking weak acid-responsive hydrophilic polymer onto the mBiosome via bioorthogonal chemistry. The results show that the reBiosome exhibits reduced virus-like immunogenicity, prolonged blood circulation time and enhanced gene delivery efficiency to weakly acidic foci (like tumor and arthritic tissue). Furthermore, reBiosome demonstrates robust therapeutic efficacy in breast cancer and arthritis by delivering gene editing and silencing systems, respectively. In conclusion, this study develops a universal, safe and efficient platform for gene therapies for cancer and inflammatory diseases.
Identifiants
pubmed: 37572220
doi: 10.1007/s40820-023-01153-y
pii: 10.1007/s40820-023-01153-y
pmc: PMC10423197
doi:
Types de publication
Journal Article
Langues
eng
Pagination
197Informations de copyright
© 2023. Shanghai Jiao Tong University.
Références
Nature. 2008 Jan 31;451(7178):591-5
pubmed: 18235503
Int J Mol Sci. 2022 Aug 02;23(15):
pubmed: 35955711
J Exp Med. 1990 May 1;171(5):1797-802
pubmed: 2185333
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3652-6
pubmed: 8170963
Structure. 1995 Jul 15;3(7):645-8
pubmed: 8591041
J Control Release. 2022 Dec;352:793-812
pubmed: 36343761
Mol Cell. 2018 Sep 6;71(5):825-840.e6
pubmed: 30100266
Bio Protoc. 2015 Oct 20;5(20):
pubmed: 26539560
J Virol. 2009 Jan;83(1):440-53
pubmed: 18971266
Cancer Res. 2019 Jul 1;79(13):3155-3162
pubmed: 31171526
J Nanobiotechnology. 2021 Feb 25;19(1):59
pubmed: 33632278
Adv Virus Res. 2017;97:1-60
pubmed: 28057256
J Immunol. 2002 Apr 1;168(7):3195-204
pubmed: 11907072
Rheumatology (Oxford). 2002 Mar;41(3):329-37
pubmed: 11934972
Trends Immunol. 2001 Feb;22(2):83-7
pubmed: 11286708
Nat Commun. 2018 Mar 12;9(1):1029
pubmed: 29531262
Mol Ther Nucleic Acids. 2022 Aug 24;29:836-851
pubmed: 36159594
Int J Biol Macromol. 2023 Jan 1;224:797-809
pubmed: 36283555
Mol Ther. 2000 Sep;2(3):218-22
pubmed: 10985952
Signal Transduct Target Ther. 2021 Feb 8;6(1):53
pubmed: 33558455
Nat Rev Immunol. 2002 Sep;2(9):675-87
pubmed: 12209136
Chem Rev. 2018 Feb 28;118(4):1691-1741
pubmed: 29319301
Cytokine Growth Factor Rev. 1998 Sep-Dec;9(3-4):277-85
pubmed: 9918125
Nat Rev Genet. 2014 Aug;15(8):541-55
pubmed: 25022906
Nanomicro Lett. 2023 Feb 8;15(1):44
pubmed: 36752939
Proteins. 2008 Apr;71(1):320-35
pubmed: 17932929
Bioconjug Chem. 2019 Feb 20;30(2):263-272
pubmed: 30452233
Methods Mol Biol. 2011;807:141-57
pubmed: 22034029
Viruses. 2020 Sep 11;12(9):
pubmed: 32933033
Science. 2021 Aug 20;373(6557):882-889
pubmed: 34413232
J Control Release. 2012 Jun 10;160(2):117-34
pubmed: 22484195
J Virol. 2011 Jan;85(2):776-83
pubmed: 20980499
PLoS Pathog. 2020 Mar 9;16(3):e1008383
pubmed: 32150590
J Immunol. 2002 Jan 15;168(2):562-8
pubmed: 11777947
Nat Protoc. 2007;2(5):1269-75
pubmed: 17546023
EMBO Mol Med. 2013 Nov;5(11):1642-61
pubmed: 24106209
J Virol. 2001 Nov;75(21):10170-8
pubmed: 11581385
J Immunol Res. 2017;2017:6104054
pubmed: 28316998
Science. 2007 Feb 9;315(5813):843-8
pubmed: 17289996
Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7306-11
pubmed: 23589850
Bioact Mater. 2021 Oct 07;11:1-14
pubmed: 34938908
Adv Drug Deliv Rev. 2016 Apr 1;99(Pt A):28-51
pubmed: 26456916
Biochemistry. 2006 Mar 7;45(9):2761-6
pubmed: 16503630
Structure. 2007 Feb;15(2):215-24
pubmed: 17292839
Adv Drug Deliv Rev. 2020;159:344-363
pubmed: 32622021
Drugs Today (Barc). 2006 Sep;42(9):599-604
pubmed: 17028669
Nat Rev Immunol. 2010 Nov;10(11):787-96
pubmed: 20948547
J Virol. 1999 Dec;73(12):10447-57
pubmed: 10559363