Permeation kinetics of dimethyl sulfoxide in porcine corneoscleral discs.

The cornea is the transparent tissue in front of the eye that bends light to help the eye focus. More than five million people's vision can be restored by a corneal transplant (keratoplasty), but there is a scarcity of suitable donor tissue. Cryopreservation could potentially increase the on-demand availability of corneas by reducing expiration and contamination during hypothermic storage, and allow equitable distribution. Understanding the transport of water and cryoprotectants across the tissue is important in developing effective cryopreservation protocols. Here, we first measured the shrinking and swelling kinetics at 22 °C and 0 °C of porcine corneoscleral discs when exposed to phosphate-buffered saline and to a cryoprotectant vehicle solution containing 2.5% chondroitin sulfate and 1% dextran. Other valuable measurements were made including the density and osmolality of the vehicle solution at 0 °C, and the water fraction of porcine cornea and sclera. Using the knowledge gained from this first part to minimize background swelling, we then examined permeation kinetics of dimethyl sulfoxide (Me

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Declaration of competing interest The authors declare no conflicts of interest.