MSC.sensor: Capturing cancer cell interactions with stroma for functional profiling.
Biosensor
Drug synergy
Ex vivo drug screen
Leukemia
Mesenchymal stromal cell
Synthetic Notch receptor
Journal
SLAS discovery : advancing life sciences R & D
ISSN: 2472-5560
Titre abrégé: SLAS Discov
Pays: United States
ID NLM: 101697563
Informations de publication
Date de publication:
11 Aug 2023
11 Aug 2023
Historique:
received:
23
05
2023
revised:
20
07
2023
accepted:
09
08
2023
pubmed:
13
8
2023
medline:
13
8
2023
entrez:
12
8
2023
Statut:
aheadofprint
Résumé
Mesenchymal stromal cells (MSCs) contribute to the microenvironment regulating normal and malignant hematopoiesis, and thus may support subpopulations of cancer cells to escape therapeutic pressure. Here, we engineered bone marrow MSCs to express a synthetic CD19-sensor receptor to detect and display interacting primary CD19+ leukemia cells in coculture. This implementation provides a versatile platform facilitating ex vivo drug response profiling of primary CD19+ leukemia cells in coculture with high-sensitivity and scalability.
Identifiants
pubmed: 37573011
pii: S2472-5552(23)00061-8
doi: 10.1016/j.slasd.2023.08.003
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.