Hormone immunolabeling in resin-embedded Arabidopsis tissues.
Antibody
Model Organisms
Molecular Biology
Plant Sciences
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
12 Aug 2023
12 Aug 2023
Historique:
received:
17
05
2023
revised:
26
06
2023
accepted:
26
07
2023
medline:
13
8
2023
pubmed:
13
8
2023
entrez:
13
8
2023
Statut:
aheadofprint
Résumé
Here, we present a protocol for immunolabeling of molecules in Arabidopsis tissues. We describe steps for tissue fixation and embedding in resin of microtome-derived sections, immunolabeling using fluorescent and non-fluorescent secondary antibodies, and visualization of cytokinin and auxin molecules. This protocol is suitable to study reproductive structures such as inflorescences, flowers, fruits, and tissue-culture-derived samples. This protocol is useful for studying the distribution of a wide range of molecules including hormones and cell wall components. For complete details on the use and execution of this protocol, please refer to Herrera-Ubaldo et al. (2019).
Identifiants
pubmed: 37573503
pii: S2666-1667(23)00481-1
doi: 10.1016/j.xpro.2023.102514
pmc: PMC10448208
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102514Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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