The two-step cargo recognition mechanism of heterotrimeric kinesin.
Cargo binding
Intracellular transport
KIF3
Kinesin
Protein dynamics
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
06 11 2023
06 11 2023
Historique:
revised:
21
07
2023
received:
23
01
2023
accepted:
03
08
2023
pmc-release:
14
08
2024
medline:
7
11
2023
pubmed:
14
8
2023
entrez:
14
8
2023
Statut:
ppublish
Résumé
Kinesin-driven intracellular transport is essential for various cell biological events and thus plays a crucial role in many pathological processes. However, little is known about the molecular basis of the specific and dynamic cargo-binding mechanism of kinesins. Here, an integrated structural analysis of the KIF3/KAP3 and KIF3/KAP3-APC complexes unveils the mechanism by which KIF3/KAP3 can dynamically grasp APC in a two-step manner, which suggests kinesin-cargo recognition dynamics composed of cargo loading, locking, and release. Our finding is the first demonstration of the two-step cargo recognition and stabilization mechanism of kinesins, which provides novel insights into the intracellular trafficking machinery.
Identifiants
pubmed: 37575008
doi: 10.15252/embr.202356864
pmc: PMC10626431
doi:
Substances chimiques
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e56864Informations de copyright
© 2023 The Authors.
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