Dry skin Proliferative basal cells scRNA-seq

Journal

Journal of pharmaceutical analysis
ISSN: 2214-0883
Titre abrégé: J Pharm Anal
Pays: China
ID NLM: 101579451

Informations de publication

Date de publication:
Jul 2023
Historique:
received: 08 12 2022
revised: 04 04 2023
accepted: 06 04 2023
medline: 14 8 2023
pubmed: 14 8 2023
entrez: 14 8 2023
Statut: ppublish

Résumé

Pathological dry skin is a disturbing and intractable healthcare burden, characterized by epithelial hyperplasia and severe itch. Atopic dermatitis (AD) and psoriasis models with complications of dry skin have been studied using single-cell RNA sequencing (scRNA-seq). However, scRNA-seq analysis of the dry skin mouse model (acetone/ether/water (AEW)-treated model) is still lacking. Here, we used scRNA-seq and in situ hybridization to identify a novel proliferative basal cell (PBC) state that exclusively expresses transcription factor CUT-like homeobox 1 (Cux1). Further in vitro study demonstrated that Cux1 is vital for keratinocyte proliferation by regulating a series of cyclin-dependent kinases (CDKs) and cyclins. Clinically,

Identifiants

pubmed: 37577389
doi: 10.1016/j.jpha.2023.04.004
pii: S2095-1779(23)00065-5
pmc: PMC10422139
doi:

Types de publication

Journal Article

Langues

eng

Pagination

745-759

Informations de copyright

© 2023 The Authors.

Déclaration de conflit d'intérêts

The authors declare that there are no conflicts of interest.

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Auteurs

Minhua Huang (M)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Ning Hua (N)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Siyi Zhuang (S)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Qiuyuan Fang (Q)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Jiangming Shang (J)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Zhen Wang (Z)

Laboratory Medicine Center, Allergy Center, Department of Transfusion Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, 310000, China.

Xiaohua Tao (X)

Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, 310000, China.

Jianguo Niu (J)

Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, 750000, China.

Xiangyao Li (X)

Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Peilin Yu (P)

Department of Toxicology, and Department of Medical Oncology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Wei Yang (W)

Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China.
Laboratory Medicine Center, Allergy Center, Department of Transfusion Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, 310000, China.
MOE Frontier Science Center for Brain Research and Brain-Machine Integration, Zhejiang University School of Medicine, Hangzhou, 310000, China.

Classifications MeSH